Banca de DEFESA: TALITA COSTA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TALITA COSTA DOS SANTOS
DATA : 30/07/2020
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:

Evaluation of anti-inflammatory, antinociceptive, antioxidant and toxicological activity of the ethanolic extract and bark fractions of Peltogyne pauciflora Benth. 

PALAVRAS-CHAVES:

Peltogyne pauciflora Benth. Imburanhê. Antinociceptive. Anti-inflammatory. Antioxidant.

PÁGINAS: 108
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Farmacologia Geral
RESUMO:

Peltogyne pauciflora Benth (Leguminosae), is a caatinga plant in Brazil, popularly known as buranhê and imburanhê, and the infusion of the bark is used to treat "inflammation and pain in the spine". The objective of this study was to evaluate the ethanolic extract of P. pauciflora bark and its fractions regarding its chemical constitution, anti-inflammatory, antinociceptive, antioxidant and toxicological activity. The bark was collected in the Contendas Sincorá National Forest and were macerated in ethanol, obtaining the ethanolic extract (EEPP, doses 50, 100 and 200 mg/kg), being later partitioned, originating the hexane (H), dichloromethane ( DM), ethyl acetate (AE) and butanol (B) fractions, all the doses at 50 mg/kg. EEPP, H and DM fractions were analyzed by GC-MS, and carbohydrates, fatty and phenolic compounds were identified, being highlighted the bioactive substances: beta-sitosterol, campesterol, lupenone, lupeol, 3-methoxy-4 acid hydroxybenzoic acid, phytol, oleic acid, ethyl linoleate and palmitic acid. EEPP, H, DM and AE fractions contain high content of total phenolics. For anti-inflammatory, antinociceptive and antioxidant tests, adult male Balb/c mice were used. In abdominal contortion test induced by 0.6% acetic acid, EEPP (50 and 100 mg/kg), H, DM and AE fractions reduced the nociceptive responses in relation to the vehicle group (VH) (p <0.05). In the nociception test induced by intraplantar injection of 1.5% formalin, EEPP (50 and 100 mg/kg), H and DM fractions reduced the number of flinches in both phases (neurogenic and inflammatory), and AE fraction reduced only in the inflammatory phase in relation to VH (p <0.05), and besides, the paw edema was reduced by EEPP (100 mg/kg). In the Von Frey test, EEPP (50 and 100 mg/kg), DM and AE fractions reduced hypernociception in relation to VH (p <0.05). Myeloperoxidase production by neutrophils decreased with EEPP (50, 100, 200 mg / kg), H, DM and AE fractions compared to VH (p <0.05), as well as the lipid peroxidation determined by the test of reactive substances thiobarbituric acid (TBARS) (p <0.05). EEPP (50, 100 and 200 mg/kg) and H, DM and AE fractions decreased the expression of the TNF-α and IL-6 genes, and only EEPP (200 mg/kg) reduces the expression of the IL-17 gene (p <0.05). EEPP (100 mg/kg), H and DM fractions reduced the vascular permeability in the Evans blue test (p <0.05). EEPP (50, 100 and 200 mg/kg), H and DM fractions reduced the levels of neutrophils in the peritoneal cavity (p <0.05), as well as reducing the levels of nitrite production (p <0.05). In the lethality test against Artemia salina L., EEPP presented moderate toxicity and the fractions were considered inactive. This is the first phytochemical and biological study report of the bark ethanolic extract of P. pauciflora and its H, DM and AE fractions, which have antioxidant, antinociceptive and anti-inflammatory activities, being a promising natural source for clinical use and identification of new compounds. In the future, studies will be carried out to identify bioactive compounds and mechanisms of action, in addition to toxicological assessment with determination of the lethal dose in vivo.

MEMBROS DA BANCA:
Presidente - 1553274 - REGIANE YATSUDA
Interno - 1561874 - LUCAS MIRANDA MARQUES
Interno - 1552395 - TELMA DE JESUS SOARES
Externo ao Programa - 1788171 - BRUNO OLIVEIRA MOREIRA
Externo à Instituição - ÉRIKA PEREIRA DE SOUZA