Banca de DEFESA: MAIARA RAULINA DE JESUS DIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MAIARA RAULINA DE JESUS DIAS
DATA : 31/01/2020
HORA: 08:30
LOCAL: Instituto Multidisciplinar em Saúde / UFBA
TÍTULO:

Effects of exercise on morphological and functional changes in the pancreas and liver in an animal model of aging and obesity.

PALAVRAS-CHAVES:

Aging. Hepatic steatosis. Oxidative stress. Aerobic exercise. Inflammation. Obesity.

PÁGINAS: 92
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
RESUMO:

Objectives: To evaluate the effects of preventive and therapeutic exercises on pancreatic and hepatic morphology and function of Wistar rats in an aging and obesity model. Methods: Twenty four male Wistar rats (initial age = 4 months and final age = 14 months) were randomly assigned to three experimental aged and obese groups (n = 8 / group): sedentary, therapeutic exercise, preventive exercise. Body composition parameters, plasma concentration and immunostaining of pancreatic insulin, markers of tissue inflammation, lipid peroxidation, activity and immunostaining of antioxidant enzymes and parameters of pancreatic and hepatic structure were evaluated. Results: Only preventive exercise improved the parameters of body composition and plasma insulin concentration. Pancreatic histology showed that therapeutic and preventive exercises increased the density of pancreatic islets, but had no effect on the islet area and smaller diameter. Analysis of pancreatic and hepatic parameters indicated that only preventive exercise reduced macrophage infiltration into the pancreas and both therapeutic and preventive exercise reduced macrophage infiltration in the liver. In addition, preventive and therapeutic exercise animals had lower immunostaining for insulin Factor Nuclear Kappa B (NF-kB) and Transforming Growth Factor Beta (TGF-b) in the pancreatic parenchyma, as well as lower hepatic and pancreatic tissue lipid peroxidation, with the effect being significantly higher in the preventive exercise group. Both exercise protocols promoted smaller area of fibrosis, increased activity of catalase and Glutathione peroxidase (GPx) enzymes and increased immunostaining for Heme oxygenase 1 (HO-1) in both pancreas and liver, and preventive exercise had a greater effect on these variables. Macrovesicular steatosis was lower in animals submitted to both types of exercise protocol; however, microvesicular steatosis was not different between experimental groups. Conclusion: The beneficial effects of exercise, especially preventive exercise, on body adiposity, insulinemia, morphology, inflammation and redox stress in the liver and pancreatic parenchyma under combined conditions of aging and obesity are promising in preventing degenerative processes in these organs under both conditions.

MEMBROS DA BANCA:
Presidente - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Externo ao Programa - 2315391 - FABRICIO FREIRE DE MELO
Externo à Instituição - RAILDO DA SILVA COQUEIRO - UESB
Interno - 2497915 - ROBSON AMARO AUGUSTO DA SILVA