Banca de DEFESA: THIAGO MACÊDO LOPES CORREIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : THIAGO MACÊDO LOPES CORREIA
DATA : 17/09/2019
HORA: 09:00
LOCAL: INSTITUTO MULTIDISCIPLINAR EM SAÚDE
TÍTULO:

Effects of physical training on protection against cigarette smoke-induced oxidative stress and muscle inflammation.

PALAVRAS-CHAVES:

Exercise. Cigarette Smoke. Redox balance. inflammation.

PÁGINAS: 72
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
SUBÁREA: Fisiologia do Esforço
RESUMO:

Objective: To investigate the effects of physical training on changes in physical performance, antioxidant capacity and gene expression of inflammatory markers in skeletal muscle of mice exposed to cigarette smoke (CS). Methods: Thirty-eight eightweek-old male C57 / BL6 mice were randomly divided into four groups (n = 8 / group), submitted or not to CS and physical exercise: sedentary (SED), SED + CS, trained (TRE). and TRE + CS. All animals underwent the maximum progressive swimming test to assess aerobic capacity and inverted screen test for muscle endurance assessment. Total body mass (TBM) and food intake were verified weekly throughout the experiment. After euthanasia, visceral adipose tissue (TAV) deposits and gastrocnemius muscles were collected and weighed. TAV was used to calculate adiposity index and muscle were for glutathione peroxidase and catalase activity assays and expression of genes encoding protein markers of inflammation. Results: Food intake increased between the initial (1st to 4th week) and final (5th to 8th week) measurements in all groups. The comparison between the groups indicated that the mice exposed to cigarette smoke had lower food consumption when compared to the SED group, with no statistical difference compared to the TRE group. There was a reduction in TBM between the initial and final measurements only in the groups exposed to cigarette smoke (SED + CS and TRE + CS). Comparison of TBM between groups showed no significant difference between them. Carcass weight and adiposity index were lower in the SED + CS group compared to the other groups. Forced swim test performance improved between initial and final measurements in all groups. The comparison between the groups showed that the TRE group presented better performance when compared to the other groups; On the other hand, the SED + CS group presented worse performance when compared to the other groups, while the SED and TRE + CS groups did not present statistical difference. Performance in the inverted screen test improved between initial and final measurements in the TRE group only. The comparison between the groups showed that the TRE group presented better performance when compared to the other groups, while the SED, SED + CS and TRE + CS groups did not present statistical difference. Catalase enzyme activity was statistically higher in the TRE group compared to the other groups. For GPx enzyme, no significant difference was observed between the groups. Gene expression analysis indicated that, compared to the other groups, animals from the SED + CS group expressed more TNF-α and MCP-1 and less HO-1. The expression of the IL-10 cytokine gene was higher in the TRE group when compared to the SED and SED + CS groups, while the TGF-β gene expression was higher in the SED + CS group when compared to the TRE group. There was no statistical difference between groups in the expression of IL-1β and IL-6 cytokine-related genes. Conclusion: Four weeks of exposure to cigarette smoke induced a reduction in food intake, accompanied by greater impairment of body mass and composition, as well as reduced physical performance and altered gene expression associated with maintaining or controlling inflammation. In contrast, regular exercise protected animals exposed to smoke from changes in body composition, physical performance, and expression of the TNF-α, MCP-1, and HO-1 genes.

MEMBROS DA BANCA:
Interno - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Externo à Instituição - RAFAEL PEREIRA DE PAULA - UESB
Externo à Instituição - JOSÉ AILTON OLIVEIRA CARNEIRO - UESB