Banca de DEFESA: LORENA LOBO BRITO MORBECK

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LORENA LOBO BRITO MORBECK
DATA : 18/12/2018
HORA: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TÍTULO:

Evaluation of anti-inflammatory, antinociceptive, antioxidant and toxicological activity of the ethanolic extract and of the stem and bark fractions of Aeschynomene martii Benth.

PALAVRAS-CHAVES:

Aeschynomene martii Benth, Tripa de Galinha, antinociceptive, anti-inflammatory, antioxidant

PÁGINAS: 93
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Etnofarmacologia
RESUMO:

The plant species to be studied, Aeschynomene martii Benth, of the genus Aeschynomene, family of Leguminosae, possess bioactive compounds with great pharmacological potential to be used. The objective of this study was to evaluate the antinociceptive, anti-inflammatory, antioxidant and toxicological activities of A. martii Benth stem and bark, collected in the Contendas do Sincorá National Forest region (FLONA). The ethanolic extract was prepared by maceration and subjected to partition extraction. After evaporation, the four fractions of the crude extract were obtained in the following yields: 13.21% (hexane), 40.92% (dichloromethane), 30.68% (ethyl acetate) and 8.10% (butanol) relative to the weight of the crude extract 17.03 g. The phytochemical exploration of the fractions was performed by CCD with specific developers. Fractions of hexane and dichloromethane had their chromatographic profiles evaluated by GC-MS, with the emphasis on the identification: stigmasterol, b-sitosterol, lupenone, 3,3',4',5,5',7-hexamethoxyflavone, besides the acids fatty acids. The antioxidant activity was evaluated by the DPPH free radical sequestration test, the ethanolic extract and the fractions showed no significant antioxidant activity (p < 0.05), besides being classified as inhibitory concentration (good, medium and low, respectively). For the antinociceptive and anti-inflammatory assays adult male Balb/c mice were used. The antinociceptive effect was evaluated by the following tests: 0.6% acetic acid-induced abdominal contortion, where all fractions (25 mg/kg) promoted a significant reduction in nociceptive responses (p < 0.05). Intraplantar injection of formalin 1.5%, in phases 1 and 2, the fractions (25 mg/kg) reduced the number of flinches in comparison to the negative control (p < 0.05), when compared to the positive control group morphine, dichloromethane and butanol fractions did not present statistical difference (p < 0.05), the paw edema formed significantly reduced in the fractions (25 mg/kg) of hexane, dichloromethane and ethyl acetate (p < 0.05). In the electronic Von Frey by intraplantar injection of carrageenan (Cg), all fractions (25 mg/kg) were able to significantly reduce hypernociception (p < 0.05). For the determination of anti-inflammatory activity, tests were performed: Neutrophil migration to the peritoneal cavity, where all fractions (25 mg/kg) showed a significant reduction in neutrophil migration in (p < 0.05); Determination of nitrite levels, all fractions (25 mg/kg) presented a significant reduction (p < 0.05). Evaluation of vascular permeability by Evans blue, which showed a significant reduction of dye extravasation in the peritoneal fluid (p < 0.05). To determine the toxicity to Artemia saline Leach, the toxicity classifications of the fractions were active in ethanolic extract and hexane, moderate in dichloromethane and inactivated in ethyl acetate and butanol. The results allow us to consider that A. martii Benth is a promising natural source for the identification of novel bioactive compounds and therapeutic agents, as well as a promising species for use as a herbal remedy.

MEMBROS DA BANCA:
Presidente - 1553274 - REGIANE YATSUDA
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo à Instituição - ÉRIKA PEREIRA DE SOUZA