Banca de DEFESA: JULIA DE OLIVEIRA BORGES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIA DE OLIVEIRA BORGES
DATE: 27/02/2024
TIME: 09:00
LOCAL: IMS
TITLE:

EFFECTS OF DIETARY COMPOSITION AND/OR PHYSICAL TRAINING ON IRISIN CONCENTRATION AND MORPHOFUNCTIONAL PARAMETERS OF ADIPOSE TISSUE IN OBESE WISTAR RATS

KEY WORDS:

Obesity. High protein diet. Physical training. Adipose tissue. Irisin.

PAGES: 121
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUMMARY:

Objective: To evaluate the effects of physical training and a carbohydrate-restricted and high-protein diet on the modulation of plasma and muscle irisin and morphological and functional changes in white and brown adipose tissue of male Wistar rats in an obesity model. Methods: 40 male Wistar rats were divided into two groups: Control group (n=6) and Group with a high-calorie diet (HC) to induce obesity (n=34) for 8 weeks. After this period, 6 animals from each group were euthanized to confirm obesity. Then, the obese animals were divided into 4 groups: Group submitted to the HC diet and sedentary (n=7); Group submitted to the HC diet and trained (n=7); Group submitted to a high-protein, carbohydrate-restricted
(HP) diet and sedentary; Group submitted to a high-protein and carbohydrate-restricted diet (HP) and trained (n=7). After a period of 12 weeks, the animals were euthanized and the subcutaneous, epididymal and brown adipose tissues, as well as muscle tissue and thoracic blood were collected for further analysis. Body composition, serum and tissue modulation of irisin/FNDC5, morphology of the gastrocnemius muscle and subcutaneous (TASC) and brown (TAM) adipose tissues were evaluated. Mitochondrial activity and energy metabolism parameters in TASC and TAM and inflammatory markers, oxidative damage and antioxidant enzyme activity in TASC, TAE and TAM were also evaluated.Statistical analyzes were performed using the two-way ANOVA test (2 diets x 2 training conditions), followed by the Bonferroni post-test. Data were presented as mean±standard error. Results: The HP diet and physical training positively influenced body composition parameters, TASC and muscle morphology, TASC metabolism parameters and inflammatory parameters of the evaluated tissues. However, the effect of physical training was superior to the HP diet by protecting the trained animals from changes in the redox balance and inflammatory profile in a more pronounced way, in addition to determining the increase in the serum concentration of irisin. Conclusion: In the model studied, the effects of changes in dietary composition and physical training were decisive in protecting animals from changes caused by obesity on parameters of body composition, tissue morphology and metabolism, and the inflammatory and oxidative profile of adipose tissues. Our findings reinforce the importance of regular physical training by determining a higher concentration of circulating irisin and positive modulation in the redox balance and inflammatory profile of all tissues evaluated.

COMMITTEE MEMBERS:
Interna - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Externo à Instituição - Luciano Evangelista dos Santos Filho
Presidente - ***.988.236-** - RAFAEL PEREIRA DE PAULA - UESB
Interna - 1552395 - TELMA DE JESUS SOARES