Banca de DEFESA: EVERTON ALMEIDA SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EVERTON ALMEIDA SOUSA
DATE: 11/08/2023
TIME: 09:00
LOCAL: Instituto Multidisciplinar em Saúde-UFBA (remoto)
TITLE:

Effects of therapeutic or preventive training on function, morphology and redox balance in brown adipose tissue in an animal model of aging and obesity 

KEY WORDS:

Adipose tissue. Oxidative stress. Therapeutic exercise. Preventive exercise.

 

PAGES: 48
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia do Esforço
SUMMARY:

Objective: To evaluate the effects of therapeutic or preventive physical training on morphological and functional parameters in brown adipose tissue in an animal model of aging and obesity. Methods: Twenty-four male Wistar rats (initial age = 4 months and final age = 14 months) were randomly distributed into three elderly and obese experimental groups (n= 8/group): sedentary (EOS), therapeutic exercise (EOT) and preventive exercise (EOP). Body adiposity parameters were evaluated and, in brown adipose tissue (BAT), the activity of antioxidant enzymes, TBARS and total nitrites, morphology parameters, and gene expression of redox profile markers and tissue function. Results: Preventive exercise reduced the mass of abdominal subcutaneous adipose tissue in aged and obese animals. Total visceral abdominal adipose tissue (TAV) weight reduces in the EOT and EOP exercised groups. In the TAM, the exercise protocols, therapeutic and preventive, determined the increase in the number of adipocytes per field, reduction in the number of unilocular adipocytes, and increase in the number of multilocular adipocytes. The TAM's percentage of lipid vacuoles per field was reduced only in the EOP group. TBARS and total nitrite levels were reduced by therapeutic and preventive exercise protocols, with the reduction being more remarkable in the EOP group. The activity of the antioxidant enzyme catalase was higher in animals from the EOT and EOP groups. The activity of the GPx enzyme increased only in the preventively trained animals, although the immunostaining for GPx was higher in both the EOT and EOP groups. The analysis of the expression of the genes that encode the enzymes Catalase, GPx-1, and SOD-1 in brown adipose tissue revealed that both therapeutic and preventive exercise increased the gene expression of the antioxidant enzymes, Catalase and GPx-1, with a more significant effect of preventive training. No differences were observed between groups in SOD-1 enzyme gene expression. The gene encoding the UCP-1 protein expression in brown adipose tissue increased in the EOP and EOT groups, while the expressions of PGC-1a and SIRT 1 increased only in the EOP. Conclusion: The therapeutic and preventive exercise protocols determined improved morphology, function, and oxidative stress parameters in the brown adipose tissue of aged rats with diet-induced obesity. The preventive protocol's beneficial effects were more evident, indicating the role of regular exercise practice in attenuating or reversing alterations in brown adipose tissue in the studied animal model.

COMMITTEE MEMBERS:
Presidente - 1112111 - AMELIA CRISTINA MENDES DE MAGALHAES GUSMAO
Interna - 1552395 - TELMA DE JESUS SOARES
Externo à Instituição - LUCIANO EVANGELISTA DOS SANTOS FILHO