Banca de DEFESA: SARAH ROCHA LIMA BRAGA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : SARAH ROCHA LIMA BRAGA
DATE: 27/07/2023
TIME: 14:00
LOCAL: Instituto Multidisciplinar em Saúde
TITLE:

Evaluation of anti-inflammatory, antinociceptive, and toxicological activity of the ethanolic bark extract of Commiphora leptophloeos (Mart)

KEY WORDS:

Commiphora leptophloeos (Mart). Umburana fêmea. Antiinflammatory. Antinociception.

PAGES: 126
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUBÁREA: Farmacologia Geral
SUMMARY:

The plant species to be studied is Commiphora leptophloeos (Mart.) J.B. Gillet, popularly known as umburana fêmea of the genus Commiphora, family Burseraceae, is an endemic species of the region of the village of Palmeiras, district of the city of Contendas do Sincorá - BA. According to the local population, it is traditionally used as a tonic, healing wounds and against coughs, bronchitis, gastritis, ulcers and chronic conditions. The study aimed to evaluate the medicinal potential of this plant as an antiinflammatory, antinociceptive, antioxidant and toxicological action. The ethanolic extract was prepared by maceration and after evaporation the crude extract was obtained with a yield of 2.2%. The phytochemical prospection was carried out by CCD with specific developers and the chromatographic profile evaluated by GC-MS, highlighting the identification of: stigmasterol, lupeol, protocatechuic acid, lanosterol, ethyl oleate and ethyl linoleate. The antioxidant assay was carried out by evaluating the total antioxidant capacity, using the phosphomolybdate method, in which the plant extract showed antioxidant capacity. For nociception and inflammation assays, adult male Balb/c mice were used. The antinociceptive effect was evaluated by the tests: Abdominal writhing induced by 0.6% acetic acid, where all concentrations (25, 50, 100 and 200 mg/kg) promoted a significant reduction in nociceptive responses (p < 0.05). The concentration of 100mg/kg of the ethanolic extract demonstrated that its antinociceptive effects were mediated by the cholinergic pathway (p < 0.05). Intraplantar injection of 1.5% formalin, in phase 1 and 2, all concentrations (50, 100 and 200 mg/kg) reduced the number of flinches, compared to the negative control (p < 0.05), when incidents to the morphine positive control group, the concentrations of 50 and 200 mg/kg did not show statistical difference (p < 0.05), the paw edema formed did not obtain a significant reduction in any experimental concentration (p < 0.05). In electronic Von Frey by intraplantar injection of carrageenan (Cg), all concentrations (50, 100, 200 mg/kg) were able to significantly reduce hypernociception (p < 0.05). For the experiment of anti-inflammatory activity, tests were performed: Migration of neutrophils to the peritoneal cavity, where all concentrations (50, 100, 200 mg/kg) established a significant reduction in neutrophil migration in (p < 0.05) ; The determination of nitrite levels, all concentrations (50, 100, 200 mg/kg) showed a significant reduction (p < 0.05). Evaluation of vascular permeability by Evans blue, which showed a significant reduction in dye leakage into the peritoneal fluid at all doses (50, 100, 200 mg/kg), (p < 0.05). Toxicity innovation was performed with Artemia salina Leach and the toxicity classification was highly toxic. Cytotoxicity was investigated using the MTT assay, where concentrations of 25, 50 and 100ug/kg were shown to decrease tumor cell viability (p < 0.05). The results allow us to consider that C. leptophloeos (Mart.) is a promising natural source for the identification of new bioactive compounds and therapeutic agents, as well as a promising species for use as a herbal medicine

COMMITTEE MEMBERS:
Presidente - 1553274 - REGIANE YATSUDA
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo à Instituição - RAFAEL SANTOS DANTAS MIRANDA DÓREA - FTC