Banca de DEFESA: FERNANDA KAROLINE ALMEIDA FREIRE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FERNANDA KAROLINE ALMEIDA FREIRE
DATE: 27/07/2023
TIME: 14:00
LOCAL: Sala 401 - Prédio Administrativo
TITLE:

Oxidative stress markers in the context of miscarriage associated with Ureaplasma parvum infection

KEY WORDS:

Spontaneous abortion, Ureaplasma parvum, oxidative stress

PAGES: 79
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SPECIALTY: Microbiologia Médica
SUMMARY:

Abortion is the termination of pregnancy before the 22nd gestational week or when the fetus weighs less than 500g. It can occur spontaneously or be induced and has multiple causes, including genital infections. Genital tract infections can ascend to the uterine cavity and account for 15% of early abortions and 66% of late abortions. The exacerbated inflammatory responses resulting from the infectious process disrupt gestational homeostasis, contributing to a pro-inflammatory profile and altering the oxidative state. The main objective of this study was to evaluate oxidative stress markers in the context of spontaneous abortion associated with infections by gynecologically significant microorganisms. Biological samples of placental tissue from women who experienced spontaneous abortion and women who had full-term deliveries were used. When evaluating oxidative stress enzymes, elevated levels of lipid peroxidation markers (TBARS and carbonylated protein) were identified in the abortion group, along with increased antioxidant activity by CAT and reduced GPx activity, while SOD showed an unchanged response. However, the profile of lipid peroxidation was accentuated in the abortion group infected with UP. GPx levels were reduced in the UP-infected abortion group compared to the infected control, while SOD and CAT showed no differences. The SOD/CAT ratio was reduced and the SOD/GPx ratio was increased in the UP groups compared to the non-infected groups. In terms of gene expression ratio, abortion contributed to an increase in Mn SOD/GPx, while UP infection reduced the expression of CuZn SOD/GPx and Mn SOD/GPx in both the abortion and infected control groups compared to the non-infected groups. Furthermore, nitrite levels were increased in the UP-infected abortion group. This study demonstrated that UP infection may contribute to altering the oxidative profile, suggesting that an imbalance in oxidative stress could contribute to the occurrence of spontaneous abortion associated with bacterial infection.

COMMITTEE MEMBERS:
Presidente - 2342538 - GUILHERME BARRETO CAMPOS
Interna - 2312625 - LILIANY SOUZA DE BRITO AMARAL
Externa à Instituição - CLARISSA LEAL SILVA E SOUZA