Banca de DEFESA: ANNE CAROLINE BRITO BARROSO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANNE CAROLINE BRITO BARROSO
DATE: 01/08/2023
TIME: 08:00
LOCAL: Sala 401 - Prédio Administrativo
TITLE:

Cytokine signature profile in the genital tract of adolescent men who have sex with men (MSM) associated with sexually transmitted infections (STIs).

KEY WORDS:

STI, cytokines, infection, mycoplasmas

PAGES: 79
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SPECIALTY: Microbiologia Médica
SUMMARY:

Sexually Transmitted Infections (STIs) are defined as a group of diseases caused by microorganisms transmitted mainly through sexual contact without the use of condoms. In addition to HIV, other bacterial STIs are considered epidemic in Brazil, especially gonorrhea (Neisseria gonorrhoeae), mycoplasma infections (Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum) and Chlamydia trachomatis. During STI infection, immune cells produce a series of chemical mediators such as: tumor necrosis factor alpha (TNF-α), interleukins IL1-β, IL-6, IL-10, among others, which act by orchestrating the immune response. Objective: to define the signature of in young men who have sex with men and transsexual women, correlating the results with the prevalence of STIs due to Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasmas. Methodology: This is a cross-sectional study that is part of a pilot project entitled “PrEP Study 15-19” (Title: Study of the effectiveness of Pre-Exposure Prophylaxis [PrEP]. Clinical samples were collected in duplicate from each participant , at the time of the first visit to the service. For this, they were obtained by rubbing a swab on the penile, oral and anal urethral mucosa, being stored in 5 mL of transport medium. The quantification of microorganisms was performed using the absolute quantification technique IL-1β, IL-6, IL-10 and TNF-α tumor necrosis factor assays were performed using an eBioscience ELISA kit. Results: Our sample consisted predominantly of black men who have sex with men or brown, aged between 18 and 19 years. Of these, 17.36% had one or more infections. There was an association between complete high school education and STI (p 0.045). Among the studied STIs, the prevalence of 85.3% stands out of mycoplasmas.In the evaluation of the profile of cytokines secreted between infected and non-infected, we found no statistically significant association. Matrix tests were performed among those infected with an association between higher TNF-α and IL-6 values. In the non-infected population, there is a general association between cytokines, that is, participants with lower levels showed a reduction in all cytokines studied. In this study, we had a prevalence of 4.73% of coinfections, all with at least one mycoplasma. 50% of the coinfections found were Mycoplasma hominis and Ureaplasma urealyticum. In the evaluation of the cytokine signature between mono- and co-infected individuals, we found higher levels of IL-1β in monoinfected individuals when compared to co-infected individuals and of TNF-α in monoinfected individuals, when compared to non-infected and co-infected individuals. In observing the heatmap with unsupervised clusters, two groups can be seen, one with a greater inflammatory potential, and the other with a lower potential, with emphasis on M. hominis and U. urealyticum with a high potential for an inflammatory response. The Principal Component Analysis shows the association between M. hominis infection and IL1-β secretion. Conclusion: Our study found no statistical difference between infected and uninfected young people, however, mycoplasma infection and co-infection with gonorrhea and chlamydia demonstrate an association with increased cytokines.

COMMITTEE MEMBERS:
Presidente - 2342538 - GUILHERME BARRETO CAMPOS
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externa à Instituição - ALINE TEIXEIRA AMORIM