Banca de DEFESA: CAMILA DUTRA BARBOSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAMILA DUTRA BARBOSA
DATE: 16/12/2022
TIME: 14:00
LOCAL: https://meet.google.com/aoe-rkxg-cch
TITLE:

Evaluation of the role of serotonin in the response of macrophages obtained from mice inoculated with Staphylococcus aureus and Escherichia coli

KEY WORDS:

5-HT, Serotonin, Staphylococcus aureus, Escherichia coli, in vivo, in vitro

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SPECIALTY: Microbiologia Médica
SUMMARY:

Serotonin (5-hydroxytryptamine or 5-HT) modulates immune responses in immune cells, and this action occurs through the serotonin system. This monoaminergic neurotransmitter acts on immune cells, coordinating the immune responses of macrophages, Natural killer (NK) cells, dendritic cells, and lymphocytes, either inhibiting or stimulating them. This immunomodulatory role occurs mainly in cells with pro-inflammatory action, especially the effects of 5-HT on different immunological stimuli in specific tissues that are not known. Thus, the present study aimed to evaluate the effect of 5-HT on the immune response induced by inoculation of Staphylococcus aureus and Escherichia coli in the culture of peritoneal and splenic macrophages from mice treated or not with serotonin. For this, mice of the C57BL6 strain were used. The first animal was treated with 5-HT in vivo, where the ventricular group mice were operated on by intracerebral surgery (ICV). The second group of sham animals experienced an ICV surgery; however, only 0.1% ascorbic acid (vehicle) was administered. After surgery, peritoneal and splenic macrophages were extracted from the animals and incubated for 24 hours. The sham group was divided into two subgroups: macrophages treated with 5-HT in vitro and untreated macrophages. The macrophages treated in vitro received different doses of 5-HT (10^-3 M and 10^-8 M) and were again incubated for 24 hours, while the subgroup of untreated macrophages did not undergo any contact with 5-HT. After the incubation period, respectively, negative macrophages treated in vivo, in vitro, and untreated were infected with E. coli and S. aureus, LPS (positive control), or saline (control) and incubated again for 3 hours. After collection, the gene expression of the cytokines TNF-α, IL1β, and IL-6 was performed in the cells, as well as the reactive oxygen and nitric oxide species in the supernatant. In general, macrophages from animals treated in vivo with 5-HT and macrophages treated in vitro with 10^-3 M and 10^-6 M 5-HT showed lower inflamed marker faces when compared to macrophages without any treatment, mainly for infections using E. coli and S. aureus. The same effect was not observed with in vitro treatment using 10^-8 M 5-HT. In addition, serotonin may also be used in the future as a therapeutic agent in the treatment of inflammatory diseases. However, studies are carried out for more studies carried out in this process.

COMMITTEE MEMBERS:
Interna - 1552395 - TELMA DE JESUS SOARES
Interno - 1561874 - LUCAS MIRANDA MARQUES
Externo ao Programa - 2315391 - FABRICIO FREIRE DE MELO - UFBA