Banca de DEFESA: BRUNO CAJADO ALMEIDA GOUVEIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNO CAJADO ALMEIDA GOUVEIA
DATE: 21/01/2022
TIME: 09:00
LOCAL: Virtual
TITLE:

Do sponges have a growth zone? In situ, morphological, and molecular evidences

KEY WORDS:

Transcriptome, EvoDevo, RNAseq, Developmental Biology, Archaeocyte, Choanocyte.

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Morfologia dos Grupos Recentes
SUMMARY:

In Porifera, growth is considered indeterminate and disorganized, in opposition to the preferential growth zones observed in many modular invertebrates. But are sponges that simple? Understanding whether body regionalization is present in sponges is important to comprehend the evolution of the body plans in metazoans. Here, we are investigating this issue by comparing the morphometry, histology, and the transcriptome (RNAseq, de novo) of two body regions of the repent sponge Cladocroce caelum (Haplosclerida): proximal (with developed aquiferous system modules) and distal regions (the hypothetical growth zone). We observed by in situ measurements that the growth is carried out preferentially at the distal region . We found differences in the histological composition between regions, with a predominance of choanocytes (maintenance cells, 47.6%) at the proximal region, and archaeocytes (totipotent cells, 65.3%) at the distal one. We recovered 99% of orthologous predicted genes for Eukarya and 94% for Metazoa in the transcriptome of the species. Almost all genes related to germ line and stem cells and developmental signalling pathways found in other sponges were expressed in both regions of C. caelum. However, among 140,614 transcripts, only 60 were significantly differentially expressed between regions: 21 were upregulated at the proximal region, while 39 at the distal one. Thus, the comparison of these three characteristics of between these two regions showed differences, which may indicate some regionalization in the sponge body. Our results indicate that despite the differences in the body plan of sponges compared to other invertebrates, these animals can share specialized growth zones.

BANKING MEMBERS:
Interno - 1914176 - EMILIO DE LANNA NETO
Interna - 3143542 - JANAINA LIMA DE OLIVEIRA
Externo à Instituição - FEDERICO DAVID BROWN ALMEIDA
Externo à Instituição - BRUNO COSSERMELLI VELLUTINI
Externo à Instituição - ANDRÉ QUEIROZ DE PADUA