Banca de DEFESA: AMANDA ANDRADE DO ROSÁRIO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : AMANDA ANDRADE DO ROSÁRIO
DATA : 09/06/2021
HORA: 09:00
LOCAL: Google Meet
TÍTULO:

Identification and molecular characterization of Wolbachia pipientis strains in natural populations of sandflies collected in leishmaniasis endemic areas

PALAVRAS-CHAVES:

Diptera, Psychodidae, Phlebotominae, Leishmania, Wolbachia, molecular4 identification

PÁGINAS: 52
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
SUBÁREA: Entomologia e Malacologia de Parasitos e Vetores
RESUMO:

Leishmaniasis is a neglected disease, directly associated with social and environmental factors, being present in practically all continents. It is a disease caused by protozoa of the genus Leishmania, which is transmitted to animals and humans through the bite of insects from the Psychodidae family. Among the vectors of leishmaniasis, Lutzomyia longipalpis and Nyssomyia whitmani are the main responsible for the two clinical forms of the disease in Bahia, Visceral Leishmaniasis and Leishmaniasis Tegumentar, respectively. The growing number of cases of this disease, associated to severe sequelaes and even death, has increased the search for methodologies that allow its control. One of them has been the use of biological controls that can act both in the direct fight against the disease and in the control of vectors. In this context, the use of Wolbachia pipientis, an obligatory intracellular bacterium, naturally present in 60% of insects, has been thought of as a possibility, since studies have shown that their strains can lead to different changes in the hosts, which tend to decrease their lifespan or prevent the development of parasites. This study aimed to detect and molecularly characterize the circulating strain(s) of this bacterium in sandflies in the State of Bahia, Brazil, in addition to evaluating the correlation between Wolbachia and Leishmania, in order to identify possible strategies for control alternative to diseases caused by transmitting mosquitoes, with a focus on leishmaniasis. A total of 745 sandfly specimens were collected in nine municipalities in Bahia, belonging to the two sandfly species: Lu. longipalpis and Ny. whitmani. DNA analyzes via PCR were used to detect Leishmania (ITS) and Wolbachia (16S) in the samples under study, in addition to the determination of circulating Wolbachia strains (WSP). Of the amount of specimens collected, 79.6% were identified as Lu. longipalpis (found in eight of the nine municipalities collected) and 20.4% as Ny. whitmani, the latter being present exclusively in the municipality of Wenceslau Guimarães. Natural infection by Leishmania was identified in 43.5% of the total females analyzed for both species of sandflies. The positivity of Wolbachia infection in Lu. longipalpis populations evaluated in this study represents 0.16%, while for Ny. whitmani it was 70%, with co-infection identified by Leishmania infantum and Wolbachia 37.5% of Ny. whitmani females. A single strain of Wolbachia was identified among the positive samples, being known as wStv MI or wWhi, known to lead to a phenotype of cytoplasmic incompatibility in insect vectors. No correlation between Wolbachia and Leishmania was found.

MEMBROS DA BANCA:
Presidente - 250.273.218-21 - FREDDY RUBEN BRAVO QUIJANO - UEFS
Externo à Instituição - CARLOS EDUARDO DE ALMEIDA - UFBA
Externa à Instituição - KAMILA GAUDÊNCIO DA SILVA SALES - Fiocruz - PE