Banca de DEFESA: JOÃO PAULO COTRIM GAMA DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JOÃO PAULO COTRIM GAMA DA SILVA
DATA : 30/07/2020
HORA: 09:30
LOCAL: Webconferência
TÍTULO:

IgE antibodies to recombinant core antigens ando non-structural proteins NS3, NS4A/B e NS5B of the hepatitis C vírus.

PALAVRAS-CHAVES:

Hepatitis C, IgE, recombinant antigens, cytokines

PÁGINAS: 75
GRANDE ÁREA: Ciências Biológicas
ÁREA: Imunologia
RESUMO:

In hepatitis C, HCV persistence may cause cirrhosis, liver failure and hepatocellular carcinoma. New biomarkers and the development of efficient screening tests are still necessary to diagnose seronegative anti-HCV IgG patients. Aim: The aim of this study was to investigate the adaptive immune response of anti-HCV IgE antibodies in patients chronically infected with HCV, and to test its use as a biomarker of antiviral treatment. Methods and Results: Twenty patients before and after 12 weeks of antiviral treatment with interferon-α / ribavirin took part of this study. Amplified indirect ELISAs tested IgE antibodies to recombinant antigens from core and from nonstructural proteins NS3, NS4A/B and NS5B with IgG-depleted sera. Immunocytometric assays determined serum levels of cytokines (TNF-α. IFN-γ, IL-4, IL-6 and IL-10). IgE antibodies to the HCV-recombinant antigens were detected in the hepatitis C patients. Except for NS5B IgE antibodies, there was a significant drop in the levels of IgE to Core, NS4A/B and mainly NS3 after 12 weeks of antiviral therapy regardless of the presence of an early sustained virologic response. Conclusions: These results suggest that IgE antibodies to core and non-structural proteins actively participate in the adaptive immune response to HCV infection and that anti-NS3 IgE should be investigated as a biomarker to monitor antiviral therapy and to exclude hepatitis C in anti-HCV IgG seronegative patients.  

MEMBROS DA BANCA:
Interno - 284101 - AJAX MERCES ATTA
Interno - 2138054 - ALEX JOSE LEITE TORRES
Externo à Instituição - LUIS JESUINO DE OLIVEIRA ANDRADE
Externo ao Programa - 3187360 - MARCIA CRISTINA AQUINO TEIXEIRA
Interno - 6285947 - ROBERTO JOSE MEYER NASCIMENTO