Banca de DEFESA: HONORIO DIAS ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : HONORIO DIAS ALVES
DATE: 14/06/2022
TIME: 14:00
LOCAL: SALA DA PLATAFORMA DE CONFERÊNCIA WEB RNP
TITLE:

Characterization of transgenic arabidopsis lines (Arabidopsis thaliana) overexpressing the RcNF-YB8 gene from Castor (Ricinus communis L.) under abiotic stress conditions

KEY WORDS:

Seeds, Ricinus sp., abiotic stress, drought and salinity

PAGES: 100
BIG AREA: Outra
AREA: Tecnologia e Inovação
SUMMARY:

Climate changes have a direct impact on plant agricultural production, as they lead to a gradual process of increasing abiotic stress conditions, affecting crop development and productivity. Ricinus communis (castor) is an oleaginous species of significant economic importance due to the high oil content in its seeds. Being cultivated in tropical and subtropical regions around the world. In Brazil, the castor bean represents significant socioeconomic importance as it is mainly cultivated by family farmers in the semi-arid region of the Northeast, where environmental conditions of drought and saline soils prevail. From the point of view of plant molecular physiology, transcription factors act in the regulation of development, and morphogenesis in response to environmental stresses. The nuclear factor-Y (NF-Y) belongs to the family of transcription factors (TF), being present in all eukaryotic genomes. It is a protein complex formed by three different subunits HapB (NF-YA), HapC (NF-YB), and HapE (NF-YC), each subunit being encoded for more than one gene in plants. NF-YB proteins play an important role in regulating plant responses to abiotic stresses. The present study involved the B-subunit gene (RcNF-YB8) of castor bean, which was functionally characterized in Arabidopsis (Arabidopsis thaliana) by stresses induced by osmotic restriction by imbibition and germination in saline and mannitol Endereço: Av. Reitor Miguel Calmon s/n - Vale do Canela - CEP 40110-100 - Salvador / Bahia Tel: (71) 3283-8894 Email: ppgbiotec@ufba.br Universidade Federal da Bahia Instituto de Ciências da Saúde Programa de Pós-Graduação em Biotecnologia solutions, as well as in the presence of Exogenous abscisic acid (ABA). Arabidopsis transgenic strains overexpressing RcNF-YB8 showed more vigorous germination than the wild strain (Col-0) in mannitol (400 mM and 500 mM), NaCl (200 mM and 250 mM), and ABA (1.0 µM) solutions and 2.0 µM). The antioxidant activity of the enzyme superoxide dismutase (SOD) was also analyzed. Taken together, these results suggest that RcNFYB8 induces salinity tolerance of Arabidopsis, proving to be a potentially useful molecular and biochemical marker for the genetic improvement of castor, aiming at better performance under conditions of water restriction and salinity, common in the northeastern semiarid region.

BANKING MEMBERS:
Externa à Instituição - CIMILLE GABRIELLE CARDOSO ANTUNES
Externa ao Programa - 2724248 - MARTA BRUNO LOUREIRO
Presidente - 2085555 - RENATO DELMONDEZ DE CASTRO