Banca de DEFESA: CAROLAINE ELLEN DA CRUZ HORA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLAINE ELLEN DA CRUZ HORA
DATE: 20/04/2022
TIME: 10:00
LOCAL: Salas Especiais - Qualificação/Defesa, 1º andar - ICS
TITLE:

Levana production by Zymomonas mobilis and Bacillus using low-cost carbohydrate sources

KEY WORDS:

levan, bioreactor, palm, sugar cane garapa

PAGES: 54
BIG AREA: Outra
AREA: Tecnologia e Inovação
SUMMARY:

In this research, two levan-producing microorganisms, Zymomona mobilis and a Bacillus isolated at the Laboratory of Biotechnology and Ecology of Microorganisms (LABEM) were used to evaluate the synthesis of the biopolymer through a synthetic medium rich in sucrose and two alternative sources of carbohydrates (palm and garapa). Levan is an exopolysaccharide (EPS) obtained by the transfructosylation reaction during the fermentation of cultures with Zymomonas mobilis when grown in sucrose-rich medium. This bacterium has aroused great interest by researchers and the industrial sector for presenting high yields with the productivity of ethanol and in the production of levan, when sucrose is used as a carbon source. Several researches seek to better understand the metabolic pathways of levan synthesis as well as the optimization of the process for its production. It is known that the production of levan by Zymomonas mobilis is hampered by the ability of this strain to direct its metabolism towards the production of bioproducts such as ethanol instead of the biopolymer. Two alternative sources of carbohydrates (garapa and palm) were tested in the culture medium with a concentration of 20% in the medium, the experiments were carried out in triplicates in the rotating shaker for 48 h and in the aerated biological bioreactor the best parameters in batches of 12 h. Experimental tests carried out in shaker and bioreactor using Z. mobilis and Bacillus, showed that the solution of palm and garapa used in consortium and palm separately were efficient in the formation of levan synthesis. The FTIR and RAMAN spectroscopy analyzes compared the levan produced in this research with samples of levan supplied by Sigma and demonstrated that there is a high degree of similarity between the productions with the standard gum.

BANKING MEMBERS:
Interno - 3248205 - GUSTAVO MIRANDA PIRES SANTOS
Externo à Instituição - JAILTON DE AZEVEDO SILVA JUNIOR
Presidente - 3530637 - JOSILENE BORGES TORRES LIMA MATOS