Banca de DEFESA: FILIPE SAMPAIO REIS DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FILIPE SAMPAIO REIS DA SILVA
DATA : 10/09/2021
HORA: 08:45
LOCAL: https://conferenciaweb.rnp.br/webconf/ppgbiotec-ufba
TÍTULO:

IMPLEMENTATION OF SYNTHETIC BIOLOGY PRINCIPLES FOR THE CONSTRUCTION OF ADVANCED GENETIC SYSTEMS FOR BIOTECHNOLOGY

PALAVRAS-CHAVES:

Synthetic Biology; Genetic Circuit; CRISPR, Recombinant Protein, Diagnostics

PÁGINAS: 100
GRANDE ÁREA: Outra
ÁREA: Tecnologia e Inovação
RESUMO:

Synthetic biology was defined as a specific area of knowledge in the 21st century and has already been introduced in the fields of industry, energy, agriculture and health. In its earliest days, synthetic biology was consolidated as a separate discipline from molecular biology, focusing on the engineering of new biological functions by incorporating computer sciences and electrical engineering concepts. The construction of synthetic genetic circuits with complex functionalities was the ammunition for the progress of this novel area. More recently, the CRISPR revolution in genetic engineering helped establish multiple alternative molecular tests with potential application, for example, at the point of patient care or in industrial quality control. This work was based on the hypothesis that it is possible to apply synthetic biology principles to the idealization and optimization of genetic tools that are already commonly used for biotechnological purposes, including recombinant protein expression systems and genetic biosensor devices. First, a genetic circuit was constructed to aid the expression of soluble recombinant proteins through controlled intracellular processing. This system yielded a high concentration of soluble recombinant protein without the solubilization tag was up to 67.3%. The application of this system will facilitate the production of proteins of difficult expression and of biotechnological interest, such as Bst-LF polymerase, which has application in biosensor systems. The objective is to integrate this enzyme into a diagnostic tool also developed in the context of this study, based on the CRISPR technology, which has already been adapted to detect the RNA of the SARS-CoV-2 virus. The test had an analytical sensitivity of 50 viral copies/μL and showed high accuracy in patient samples (area under the ROC curve = 1.0; CI: 0.715 - 1.00), using detection by a mobile phone application with computer vision integrated. The design of both technologies demonstrates the potential of synthetic biology for the development of biotech products and tools.

MEMBROS DA BANCA:
Externo à Instituição - Rommel Thiago Juca Ramos
Presidente - 1818609 - LUIS GUSTAVO CARVALHO PACHECO
Externa à Instituição - SILVANA BEUTINGER MARCHIORO
Externo ao Programa - 2415395 - THIAGO LUIZ DE PAULA CASTRO
Externo à Instituição - VASCO ARISTON DE CARVALHO AZEVEDO