Banca de DEFESA: JORGE LUIS NICOLETI

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JORGE LUIS NICOLETI
DATA : 30/06/2021
HORA: 14:30
LOCAL: Sala da Plataforma Conferência Web (https://conferenciaweb.rnp.br/webconf/ppgbiotec-ufba)
TÍTULO:

Determination of Corynebacterium pseudotuberculosis infection biomarkers in goats: Classical Methods, Nuclear Magnetic Ressonance and Chemometry

PALAVRAS-CHAVES:

caseous lymphadenitis, goats, metabolomic studies, chemometry

PÁGINAS: 118
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

Caseous lymphadenitis (CL), an infectious disease caused by the Gram-positive bacterium Corynebacterium pseudotuberculosis in goats and sheep, is highly prevalent in small ruminant herds worldwide. Several economic losses, represented by the decrease in the value of leather, condemnation of carcasses and reproductive failures, have already been associated with the occurrence of the disease. Currently available techniques for clinical and laboratory diagnosis of the disease lack market availability and/or sensitivity and, therefore, infected animals can remain in the herd, serving as a source of infection for other animals. Nuclear Magnetic Resonance (NMR) and chemometrics are techniques that can identify metabolites not detectable by classical laboratory diagnostic techniques. The present study aimed to determine markers of C. pseudotuberculosis infection in goats using classical methods (clinical, hematological, biochemical), serological, NMR and chemometry. A herd of 173 Canindé goats was used, from which blood and serum samples were collected for analysis. The animals were classified as uninfected control (C), asymptomatic (A) and symptomatic (S), according to microbiological isolation, based on the content of caseous lesions and serological assay (ELISA). Complete blood counts, clinical biochemistry tests were performed to determine the concentration of serum compounds; 1H NMR analyzes NOESY (Nuclear Overhauser Effect SpectrosopY) and CPMG (Carr-Purcell Meiboom-Gill) and Partial Least Square Discriminant Analysis chemometry (PLS-DA) for verification and possible biomarkers for the disease. A high dissemination of the infection by C. pseudotuberculosis was observed in the herd, with 86.13% of positive animals, being 74.57% asymptomatic and 11.56% symptomatic. In the hemogram and clinical biochemistry analyses, the only statistical difference found was the higher level of serum urea in asymptomatic individuals than in non-infected animals. In the evaluation of 62 serum samples by NMR, the techniques employed were satisfactory in discriminating the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for CL. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, opening up promising possibilities for the establishment of NMR and chemometric techniques as CL suitable diagnosis methods, both because of the advantages of the techniques and the results reported herein for the first time in goats.

MEMBROS DA BANCA:
Externo ao Programa - 2584563 - MARTINS DIAS DE CERQUEIRA
Externo à Instituição - MATEUS MATIUZZI DA COSTA
Presidente - 1561789 - RICARDO WAGNER DIAS PORTELA
Externa ao Programa - 1090486 - SILVANA BEUTINGER MARCHIORO
Externo ao Programa - 283.171.225-49 - VASCO ARISTON DE CARVALHO AZEVEDO - UFMG