Banca de DEFESA: VITOR DOS SANTOS ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : VITOR DOS SANTOS ALVES
DATA : 22/04/2021
HORA: 14:00
LOCAL: sala de conferência do PPGBiotec na plataforma Conferência Web RNP (https://conferenciaweb.rnp.br)
TÍTULO:

MULTIPLEX PCR ASSAY FOR IDENTIFICATION OF MITES IN CULTURE AND ENVIRONMENTAL SAMPLES

PALAVRAS-CHAVES:

Multiplex-PCR; Species identification; Blomia; Glycycometus; Dermatophagoides.

PÁGINAS: 60
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

It is widely accepted that some mites play a central role in triggering allergic manifestations. Among the mites, we can highlight those of the genus Blomia and Dermatophagoides as the most important cause of allergy in Brazil. Mites are routinely cultivated with the intention of producing allergenic extracts for the purpose of diagnosing and treating allergies. Currently, the identification of mites is made based on morphological characteristics; however, this technique is a time-consuming and ambiguous. To overcome these drawbacks, we developed a multiplex PCR (mPCR) assay based on ribosomal DNA (rDNA) capable of identifying the B. tropicalis, D. pteronyssinus and Glycycometus malaysiensis mite species. For this, the complete ITS2 region, flanked by partial sequences of the 5.8 S and 28 S subunits, was amplified by PCR and sequenced. The sequences obtained were aligned with sequences obtained from the Genbank database and used in the design of species-specific primers. Three pairs of primers were evaluated for their sensitivity and specificity and used to compose the mPCR. The mPCR assay was tested on environmental samples, evaluating the frequency of the mites studied in twenty dust samples from homes in the city of Salvador, northeastern Brazil. The data showed that the mites B. tropicalis, G. malaysiensis and D. pteronyssinus, were present in 95%, 70% and 60% of the collected samples, respectively. It was the first time that G. malaysiensis was identified in Brazil and that its ITS2 sequence was made available. Results demonstrate that the mPCR assay proved to be a fast, reliable and straightforward tool for identifying these mites in culture and environmental samples, and can be applied in future epidemiological or diagnostic studies for the mites studied.

MEMBROS DA BANCA:
Externo(a) ao Programa - 1260867 - BARBARA DE CASTRO PIMENTEL FIGUEIREDO
Presidente - 1927897 - CARINA DA SILVA PINHEIRO
Interno(a) - 1561789 - RICARDO WAGNER DIAS PORTELA