Characterization of recombinant proteins with antigenic and immunoprotective potential of
Corynebacterium pseudotuberculosis.
Caseous Lymphadenitis. Immunodiagnosis. Vaccines.
Caseous lymphadenitis (CLA) is a chronic infection that mainly affects sheep and goats. The use of recombinant proteins for immunoassays and vaccine production has emerged as a safe option, since it does not have the risk of pathogenic reversal. Possible virulence factors DTx, Trx, TrxR, LexA, SodC, SpaC, NanH and PknG may represent new target proteins in this approach. For the immunoenzymatic assays (ELISA), the SodC protein was selected for validation tests and epidemiological survey with serological samples of small ruminants from the semiarid state of Bahia. In the immunization test 26 goats race Canindé were used in different vaccine formulations. After immunization (0 and 30 days) and challenge (90 days), the animals were euthanized for postmortem analysis (170 days). In ELISA, recombinant SodC showed sensitivity and specificity of 96% and 94% for goats and 95.3% and 97.8% for sheep, respectively. In the epidemiological analysis with 756 sera, SodC was able to identify 95 positive samples (23%) for goats and 75 (21%) for sheep. Results superior to those obtained by the BHI antigen. In the immunization test, all animals challenged with virulent Corynebacterium pseudotuberculosis showed some reaction at the site of infection. In postmortem analysis, 33% of goats immunized with Ag-Labimuno (NanH, SodC, PknG and SpaC plus MontanideTM) showed no pathological lesion and 50% showed partial protection. 33% of the animals from the Ag-SP group (DTx, Trx, TrxR, LexA, plus MontanideTM) showed no pathological changes and 66% local lesions. In the Ag+T1 group (all antigens plus attenuated T1 strain), 20% had no lesion and 80% had lesion at the challenge site. The humoral response of animals vaccinated with the Ag-Labimuno formulation plus MontanideTM was higher than in the other groups. In the individual evaluation, NanH and SodC antigens induced a strong humoral response in the different vaccine formulations. The ELISA using rSodC protein is able to discriminate positive and negative serum samples with high levels of sensitivity and specificity. In addition, the use of SodC and NanH antigens,