Biosynthesis of silver nanoparticles by endophytic fungus Phyllosticta capitalensis
Biosynthesis, Silver Nanoparticle, Endophytic Fungus, Phyllosticta Capitalensis.
Nanotechnology is a multidisciplinary research area that comprises biology, physics, chemistry and engineering for the development, manipulation and application of materials at the nanometer scale. Although metal nanoparticles can be synthesized using a variety of physical and chemical processes, they can have some disadvantages. The biological synthesis or biosynthesis of nanoparticles can eliminate the problems caused by conventional methods and provide environmentally friendly nanoparticles. Nanoparticle biosynthesis is characterized by the use of the most diverse biological systems. However, fungi are the most efficient candidates for the manufacture of intra and extracellular metal nanoparticles. The aim of this work is to synthesize and characterize silver nanoparticles through the endophytic fungus Phyllosticta capitalensis isolated from Pyrostegia venusta and to compare two synthesis methodologies using the mycelial fragment and fungal biomass. The results showed that only the methodology I - fungal biomass has the capacity to synthesize the nanoparticles. This fact was confirmed by a color change after 72 hours of the addition of silver nitrate and also by the presence of an absorption band in the 400 nm region. The silver nanoparticles formed had spherical shapes, average size of 51.33 nm and zeta potential of -22.5 mV. Fourier transform infrared spectroscopy detected bands corresponding to vibrations of alcohol, methylene, amine, ester, nitrate residue, aliphatic amine, phosphate and aromatic hydrocarbons. These results show that the use of filtrated fungal biomass cell of Phyllosticta capitalensis at pH 9 is promising for the synthesis of silver nanoparticles.