Banca de DEFESA: ANDERSON DE JESUS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANDERSON DE JESUS SANTOS
DATA : 23/01/2020
HORA: 14:00
LOCAL: Salas Especiais - Qualificação e Defesa 1º andar, Instituto de Ciências da Saúde
TÍTULO:

Infection by Trypanosoma sp. in Boa constrictor from the northeastern region of Brazil: hematological changes and molecular characterization

PALAVRAS-CHAVES:

Wild animals, Boidae, clinical markers, trypanosomiasis,

PÁGINAS: 100
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

Among reptiles, the occurrence of Trypanosoma in snakes is rarely described, with few reports restricted to venomous species. Therefore, the present study aimed to investigate the occurrence of Trypanosoma sp. in Boa constrictor in northeastern Brazil, characterize the identified parasites and evaluate the effects of parasitism on the physiology of the animal. A total of 61 Boa constrictor were evaluated, 47 from Bahia and 14 from Sergipe, from which blood samples were obtained for blood cell count and clinical biochemistry, genomic DNA and trypanosomal isolation. Nine (15%) animals with extracellular flagellate forms were identified in blood smears, 01 in the state of Bahia and 08 in Sergipe. The parasitemia found ranged from 1% to 7%. The parasites had an exuberant undulating membrane and a small rhiniform kinetoplast anterior to the nucleus. The parasitized animals were debilitated with opaque scales and remnants of ecdysis. The comparison of hematological values between infected and uninfected animals was significant for globular volume (p <0.0005), hemoglobin (p <0.005), mean corpuscular volume (p <0.005) and mean corpuscular hemoglobin (p <0.05), with infected animals always showing lower values than uninfected animals. In leukometry, there was a difference between eosinophil (p <0.00005) and heterophil (p <0.05) counts, always higher in parasitized animals. Slow-growing pleomophilic epimastigote forms were observed on culture, measuring on average 57.04 µm in length, 3.45 µm in width and 29.47 µm of free flagellum from the kinetoplast. Sequencing of part of the 18S and GAPDH genes identified high homology with Trypanosoma serpentis. We conclude that Trypanosoma serpentis infection leads to the development of pathological process in these animals, characterized mainly by anemia

MEMBROS DA BANCA:
Externo ao Programa - 2582698 - BARBARA MARIA PARANA DA SILVA SOUZA
Externo à Instituição - ILKA BORGES BIONDI
Presidente - 1561789 - RICARDO WAGNER DIAS PORTELA
Interno - 1673868 - THIAGO CAMPANHARO BAHIENSE