Banca de DEFESA: IAGO PINHEIRO FORTUNA NUNES
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : IAGO PINHEIRO FORTUNA NUNES
DATA : 22/01/2020
HORA: 09:00
LOCAL: Salas Especiais - Qualificação e Defesa - Instituto de Ciências da Saúde
TÍTULO:
Evaluation of Antimicrobial Photodynamic Therapy Using Taylor-Associated LED Blue (λ630 ɳm ± 1 ɳm) in Candida albicans Resistant Fluconazole: In vitro Study
PALAVRAS-CHAVES:
Fungi are pathogens in humans and animals. Among the most important pathogenic fungi are yeast species that belong to the genus Candida. C. albicans can cause a wide range of diseases, including superficial mucosal infections (vulvovaginal and oropharyngeal candidiasis) that can invade life-threatening tissues. Due to the inherent characteristics of eukaryotes, seen both in fungi and in the human host, antifungal drugs mostly have low selective toxicity. This causes a considerable reduction in the antifungal therapeutic arsenal. Antimicrobial photodynamic therapy (PDT) has been advocated as an alternative to antimicrobial agents due to the extensive and inappropriate use that gradually led to the development of generalized resistance. Therefore, this study aimed to evaluate, in vitro, the efficacy of Antimicrobial Photodynamic Therapy associating Taylor Blue with a Red LED emitter (41.2 mW, λ630 ±m ± 1 ɳm, 20 J / cm2) on the culture of Candida Albicans. Assays were performed using bacterial greenhouse grown Candida albicans (ATCC 900228) strain (UltraSafe HF 212, PR, Brazil) 37◦C for 24 hours according to the fungal growth curve. The colonies were resuspended in sterile 0.85% NaCl saline at a concentration of 2 x 108 cells / mL. Three experimental protocols were performed using 750 ɳg / mL photosensitizer and 20 J / cm2 energy density, being divided into three large groups; Single AFD, double application in a single session and double application after 24 hrs, with subgroups divided into Control (C. albicans only), DMMB (photosensitizer only, TFD (factor union). The results showed respective reductions of 99, 9% and 99,991% of the total fungal load in the Protocols 3 and 2 used, being Protocol 2 the best result presented with a methodology fully compatible with clinical reality, with capacity for use in several other existing microorganisms.
PÁGINAS: 100
GRANDE ÁREA: Outra
ÁREA: Tecnologia e Inovação
RESUMO:
Fungi are pathogens in humans and animals. Among the most important pathogenic fungi are yeast species that belong to the genus Candida. C. albicans can cause a wide range of diseases, including superficial mucosal infections (vulvovaginal and oropharyngeal candidiasis) that can invade life-threatening tissues. Due to the inherent characteristics of eukaryotes, seen both in fungi and in the human host, antifungal drugs mostly have low selective toxicity. This causes a considerable reduction in the antifungal therapeutic arsenal. Antimicrobial photodynamic therapy (PDT) has been advocated as an alternative to antimicrobial agents due to the extensive and inappropriate use that gradually led to the development of generalized resistance. Therefore, this study aimed to evaluate, in vitro, the efficacy of Antimicrobial Photodynamic Therapy associating Taylor Blue with a Red LED emitter (41.2 mW, λ630 ±m ± 1 ɳm, 20 J / cm2) on the culture of Candida Albicans. Assays were performed using bacterial greenhouse grown Candida albicans (ATCC 900228) strain (UltraSafe HF 212, PR, Brazil) 37◦C for 24 hours according to the fungal growth curve. The colonies were resuspended in sterile 0.85% NaCl saline at a concentration of 2 x 108 cells / mL. Three experimental protocols were performed using 750 ɳg / mL photosensitizer and 20 J / cm2 energy density, being divided into three large groups; Single AFD, double application in a single session and double application after 24 hrs, with subgroups divided into Control (C. albicans only), DMMB (photosensitizer only, TFD (factor union). The results showed respective reductions of 99, 9% and 99,991% of the total fungal load in the Protocols 3 and 2 used, being Protocol 2 the best result presented with a methodology fully compatible with clinical reality, with capacity for use in several other existing microorganisms.
MEMBROS DA BANCA:
Presidente - 1134693 - ANTONIO LUIZ BARBOSA PINHEIRO
Externo à Instituição - JULIANA SANTOS DE CARVALHO MONTEIRO
Interno - 3065750 - LUIZ GUILHERME PINHEIRO SOARES
Externo à Instituição - SUSANA CARLA PIRES SAMPAIO - UNIME