Banca de DEFESA: TÁSSIA DE AZEVEDO GUERRA CAMPOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TÁSSIA DE AZEVEDO GUERRA CAMPOS
DATA : 06/12/2019
HORA: 09:30
LOCAL: Salas Especiais - Qualificação e Defesa - Instituto de Ciências da Saúde
TÍTULO:

"Chikungunya virus: Viral proteins and their relationship to the humoral immune response"

PALAVRAS-CHAVES:

CHIK, Protein and Immunological Profile.

PÁGINAS: 100
GRANDE ÁREA: Outra
ÁREA: Tecnologia e Inovação
RESUMO:

The concomitance of outbreaks between different arboviruses such as Dengue, Zika virus and Chikungunya virus (CHIKV) with similar symptoms is of great concern in BRAZIL especially pointing to an accurate diagnosis. This study aims the diagnosis of CHIKV in patients with acute disease by analyzing the humoral immune response and the relationship with the protein profile of CHIKV. The methodology consisted of using polymerase chain reaction (RT-PCR) technique, antibodies detection by commercially available ELISA (Enzyme-Linked Immunosorbent Assay), immuno rapid test, the analysis of protein pattern of chikv and its relationship with immune response. The results showed that the humoral immune response is mainly directed to the two viral glycoproteins E1 and E2. On the other hand, the viral presence in serum can still be detected in the presence of anti-CHIKV Immunoglobulin M, but not in the presence of anti-CHIKV IgG which invalidates the detection of viremia. The use of serological tests for commercial use showed that there was a correspondence of the results for the detection of anti-CHIKV IgM, however, for the detection of anti-CHIKV IgG the results were divergent. In conclusion, it has been shown that in individuals with acute disease, viral molecular diagnosis and the presence of anti-CHIKV IgM may coexist; however, the presence of anti-CHIKV IgG invalidates the viral presence and molecular viral diagnosis.

MEMBROS DA BANCA:
Presidente - 1311777 - SILVIA INES SARDI
Externo ao Programa - 1788701 - ADRIANO FIGUEIREDO MONTE ALEGRE
Externo à Instituição - DELLANE MARTINS TIGRE - UESB