Banca de DEFESA: DANIEL TERESKA SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANIEL TERESKA SANTOS
DATA : 29/07/2019
HORA: 13:00
LOCAL: Instituto de Ciências da Saúde
TÍTULO:

MODELING AND PRODUCTION OF BIOSPURFACTANT BY Psedomonas aeruginosa IN CULTURE CONTAINING WASTE FROM THE OIL INDUSTRY

PALAVRAS-CHAVES:

Rhamnolipid, Petrochemical wastes, EOR, MEOR, Produced Water, Crude Glycerin, Pseudomonas aeruginosa

PÁGINAS: 86
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

Ramnolipids are biosurfactants produced by Pseudomonas genus, with surface and interfacial tension reduction properties, besides being able to emulsify two insoluble phases. These bioactives present advantages to chemically way surfactants because they are more easily degraded in the environment, have low toxicity and can be produced from renewable substrates. Despite the many advantages of biosurfactants over chemical surfactants, its use is not yet widely experienced in the petrochemical industry, especially due to its high production costs. An alternative would be the industrial waste use such as produced water and crude glycerin, both waste from the petrochemical industry, which would not only lower production costs, but would also give the waste a destination and add value to them. Thus, the aim of this work was to produce ramnolipid using produced water and crude glycerin as substrates, enabling and optimizing the production process of this bioactive. For this purpose, the crude and diluted water was tested, the influence of salinity on ramnolipid production it was also evaluated and finally, the production medium was optimized in relation to the nitrogen source (where NaNO₃ sources and nitrogen extract yeast were tested at varying concentrations) and carbon source (testing various concentrations of crude glycerin). As a result, a low yield of ramnolipid production it was observed with the use of produced water, a problem that were circumvented with a residue pre-treatment. Salinity also had a negative influence in ramnolipid production, where this influence was verified from the 2% (w / v) NaCl concentration. In the nitrogen sources evaluation, there were no significant differences between the best NaNO₃ concentrations and the yeast extract. However, considering the molecule physicochemical properties as surface and interfacial tension, the yeast extract was found to be the best option, along the lines of this study. Regarding the carbon source, the concentration of 25 ml/L crude glycerin provided yields of 9.33g/L ramnolipid, very satisfactory results compared to the literature. The characterization of the molecule by FT-IR and mass spectrometry showed strong similarities in relation to the molecules produced in another studies. Thus, the use of petrochemical residues produced water and crude glycerin in ramnolipid production under the studied conditions proved a satisfactory way to use these residues.

MEMBROS DA BANCA:
Interno - 6285556 - PAULO FERNANDO DE ALMEIDA
Interno - 3530637 - JOSILENE BORGES TORRES LIMA MATOS
Externo à Instituição - EDGARD BACIC DE CARVALHO - IFBA