Banca de DEFESA: GILDEON SILVA MARQUES
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : GILDEON SILVA MARQUES
DATA : 22/03/2019
HORA: 09:00
LOCAL: Auditório da Congregação, 2º andar, Instituto de Ciências da Saúde, UFBA.
TÍTULO:
Bioprospection of Biological Activities, Phytochemistry and Cosmetics of Erythroxylum Species for purposes of Biotechnological Development.
PALAVRAS-CHAVES:
Antioxidant activity; Erythroxylum; Liposome; Bioactive substances
PÁGINAS: 100
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:
The Erythroxylaceae family consists by four genera, among which Erythroxylum is the most important, as it contains about 97% of the family species.
This genus presents important bioactive substances such as tropic alkaloids, coumarins, diterpenes, flavonoids, phenylpropanoids, steroids, tannins, triterpenes and terpenes, which have biological characteristics such as antioxidants and antibacterial, pharmacological properties as central nervous system stimulant, anti-inflammatory, antifungal, antiviral, antitumoral, in addition to medical treatments without kidney disorders, sinusitis, stomach pain, amenorrhea, flu, injury and fatigue.The genus has a wide distribution, found in greater quantity in the tropical and subtropical regions of the Americas, Africa, Southeast Asia and Oceania. Several species of this genus are unique to Brazil, some of which are restricted to the Northeast, and are found in semi-arid areas (caatinga) mainly in the states of Pernambuco, Ceará, Sergipe, Alagoas, Bahia, Piauí and Paraíba. Many of the occurrence species in the semi-arid region have not yet had their chemical and pharmacological potentials evaluated. Based on these aspects, this work aimed to determine the presence of bioactive substances and biological activities in four species of the genus Erythroxylum, evaluating leaf and stem extracts with their biotechnological potentials and cosmetic use. It was obtained 40 extracts and submitted to the lethality tests of Artemia salina, antioxidant activity (sequestration of the DPPH radical, inhibition of the oxidation of β-carotene / linoleic acid, total phenolics and total flavonoids), antibacterial activity and inhibitory activity of the enzyme acetylcholinesterase.The PCA and HCA techniques of multivariate analysis were applied allowing the differentiation by samples similarity, with formation of three distinct groups, when the results of the antioxidant activity test (DPPH) were used. In the A. salina lethality test, three extracts EAFEN, EMCEN and EMCERIII with CL50 = 1,374, 1,007 and 0,703 mg / mL, respectively, with low cytotoxicity potentials were highlighted. The EMCERIII extract had MIC of 0.25 mg / mL for E. coli and 0.50 mg / mL for S. aureus demonstrating a satisfactory antibacterial activity. For the antioxidant activity (DPPH), leaf extracts EMFERI, EAFERII, EAFERI, EHFERI and stems extracts EACERII, EACERI, EMCERII, EACEN, EMCERI and EACERIII showed the lowest IC50 values with 0.043; 0.044; 0.044; 0.050; 0.060; 0.071; 0.072; 0.077; 0.088 and 0.089 mg / mL, respectively. The EMCERIII extract was used in the preformulation process through obtaining a liposomal emulsion and incorporation into cosmetic formulations (LIC and LIG).
In the post-formulation process, the LIC maintained its integrity in the accelerated activity tests and slight increase in its antioxidant activity both in the sequestration of DPPH free radicals and in the phenolic and total flavonoid contents. In view of the above, leaves and stems extracts of the Erythroxylum species and the derived products have proved to be quite promising in the development of biotechnological products, especially the EMCERIII extract which due to its high antioxidant and antibacterial power can be used in the development of cosmetics.
MEMBROS DA BANCA:
Interno - 118.484.305-82 - LIDERCIA CAVALCANTI RIBEIRO CERQUEIRA E SILVA - UFBA
Externo à Instituição - ERIKA MARIA DE OLIVEIRA RIBEIRO - UNEB
Externo à Instituição - VITOR HUGO MIGUES - UFBA