Banca de DEFESA: MARIANE MASCARENHAS OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIANE MASCARENHAS OLIVEIRA
DATA : 25/02/2019
HORA: 09:00
LOCAL: Auditório da Congregação, 2º andar, Instituto de Ciências da Saúde, UFBA.
TÍTULO:

Determination of the protein content and toxicity of marine and freshwater microalgas for indication as food complement

PALAVRAS-CHAVES:

Bioprospecting, food supplement, amino acids

PÁGINAS: 60
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

The term malnutrition is defined by the Food and Agriculture Organization (FAO) as an abnormal physiological condition caused by inadequate, unbalanced or excessive consumption of macronutrients such as carbohydrates, lipids and proteins and/or micronutrients such as vitamins and minerals, which are necessary for the development of the organism. The present study aims to evaluate the nutritional potential of five marine microalgae: Chaetoceros calcitrans, Chaetoceros gracilis, Thalassiosira pseudonana Thalassiosira fluviatilis, Skeletonema costatum and five  freshwater microalgae: Chlamydocapsa bacillus, Ankistrodesmus fusiformis, Coelastrum microporum and Kirchneriella lunares from the Microalgae Bank of the Biology Institute atFederal University of Bahia as a possible indication of food protein complement. Initially the protein levels of each microalga were determined. For quantification, the cells were lysed and the proteins precipitated with acetone. The dosage was performed by the method of Bradford (1976) and Lowry (1951). For the toxicity tests of the algal extracts, larvae of Artemia salina were used as experimental model. The results obtained from this bioprospection indicate the freaswater species Ankistrodesmus fusiformis, Coelastrum microporum and Kirchneriella lunares as candidates to be used as food supplement, since they present higher protein levels in their biomass, 49, 47 and 41%, respectively, and do not show any toxicity, according to the test used. Among the marine species, Skeletonema costatum was the one with highest protein concentration (35%), followed by Chaetoceros gracilis and Thalassiosira pseudonana, both with 29%. The Bradford method was not suitable for protein dosing in microalgae, resulting in a sub-sizing of the production. The dosage by Lowry reflected more accurately the protein content, for all species evaluated.

 

MEMBROS DA BANCA:
Externo à Instituição - EDSON DE JESUS MARQUES - UNEB
Interno - 1674313 - SUZANA TELLES DA CUNHA LIMA
Externo à Instituição - VERA LUCIA COSTA VALE - UNEB