Banca de DEFESA: LEONARDO FREIRE SANTIAGO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LEONARDO FREIRE SANTIAGO
DATE: 14/11/2023
TIME: 08:30
LOCAL: em sala do portal Conferência Web-RNP
TITLE:

EVALUATION OF Trichuris trichiura PROTEOME AS A POTENTIAL SOURCE OF MOLECULES WITH
IMMUNOMODULATORY ACTIVITY

KEY WORDS:

Proteome, Trichuris trichiura, Immunomodulatory proteins

PAGES: 95
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Soil-transmitted helminths (STHs), such as Trichuris trichiura, represent a significant global health
challenge, particularly affecting children and leading to substantial morbidity. However, T. trichiura's
ability to manipulate the host's immune responses offers a promising avenue for identifying
biomolecules with therapeutic potential in inflammatory, allergic, and autoimmune disorders. This
study marks the inaugural comprehensive exploration of the proteome of adult male and female T.
trichiura worms using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analysis
resulted in the identification of 751 parasite proteins, with 117 found exclusively in females, 277 in
males, and 356 shared between both sexes. The proteomic analysis successfully matched around 75%
of the proteins predicted in the transcriptome.
Gene ontology analysis revealed that both male and female parasites exhibited analogous profiles in

terms of cellular components, prominently featuring intracellular structures. However, female-
exclusive proteins displayed a greater diversity of cellular components. Molecular function analysis

underscored the prevalence of hydrolytic and catalytic activities, hinting at potential enzymatic
strategies employed by T. trichiura for nutrition and modulation of the host's immune response.
Notably, several proteins with immunomodulatory potential surfaced in both genders, including a
Kunitz protease inhibitor and glutamate dehydrogenase, showcasing promise for therapeutic
applications. The recombinantly produced rc4299 protein demonstrated immunomodulatory
characteristics, notably an increase in IL-10 secretion, suggesting potential use in treating autoimmune
and allergic diseases. This study illuminates the intricacies of the T. trichiura proteome, uncovering
promising therapeutic targets and emphasizing the intricate interplay between the parasite and the
host's immune system.

COMMITTEE MEMBERS:
Externo à Instituição - SILVIO SANTANA DOLABELLA - UFS
Externo à Instituição - RODRIGO RODRIGUES CAMBRAIA DE MIRANDA - UFU
Presidente - 1927897 - CARINA DA SILVA PINHEIRO
Externa ao Programa - 1090486 - SILVANA BEUTINGER MARCHIORO - nullInterna - ***.070.425-** - SUSANA CARLA PIRES SAMPAIO DE OLIVEIRA - null