Banca de DEFESA: POLINE DOS SANTOS SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : POLINE DOS SANTOS SOUZA
DATE: 13/07/2023
TIME: 14:00
LOCAL: Auditório da Congregação, 2º andar do ICS/UFBA
TITLE:

ESTABLISHMENT OF CRYOPRESERVATION TECHNIQUES FOR THE MAINTENANCE OF THE MICROALGAE BANK OF THE INSTITUTE OF BIOLOGY – FEDERAL UNIVERSITY OF BAHIA

KEY WORDS:

Microalgae, Biobanks, Cryopreservation, Cryoprotectants.

PAGES: 65
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Microalgae are photosynthetic microorganisms found in diverse habitats and capable of producing a high number of compounds of scientific, biotechnological and economic interest. The preservation of the characteristics of microalgae in biobanks is of fundamental importance for the study of its biotechnological potential and biodiversity, however, the maintenance of microalgae in a liquid medium can affect the synthesis of compounds of importance for bioprospecting due to mutations, in addition to the risks of contamination. In order to reduce these risks, cryopreservation is an alternative for the maintenance of this material, this technique is widely used in the conservation of microbial biodiversity, which consists of exposing it to cryogenic temperatures, therefore, it is a viable resource for the maintenance of algal species, assisting in the preservation of biochemical characteristics, with a reduction in the growth rate, inbreeding and generating less damage to the genome. The cryopreservation process, however, can cause damage to cells called cryoinjuries, so the use of cryoprotectantes may be necessary. This research aimed to test and adapt cryopreservation protocols for Desmodesmus brasiliensis and Chaetoceros gracilis collected from the Microalgae Bank of the Laboratory of Bioprospection and Biotechnology of the Institute of Biology of the Federal University of Bahia, as well as to evaluate the efficiency and toxicity of cryoprotectants dimethylsulfoxide and methanol in the cryopreservation of the two species and calculate the growth rate for each species. Among the two microalgae tested in the present study, it was possible to cryopreserve D. brasiliensis so that there was growth after freezing, even without the addition of cryoprotectants, showing the highest growth rate. C. gracilis did not survive the freezing stage, so it is necessary to test new cryoprotectants and cryopreservation methodologies for the species. In view of the results, it is concluded that it is possible to cryopreserve species kept in a liquid medium in biobanks using a species-specific methodology.

COMMITTEE MEMBERS:
Externo à Instituição - EDSON DE JESUS MARQUES - UNEB
Interna - 287168 - LILIA FERREIRA DE MOURA COSTA
Presidente - 1674313 - SUZANA TELLES DA CUNHA LIMA