EVALUATION OF TFDa ASSOCIATING RED LASER LIGHT (660nm) WITH TAYLOR BLUE PHENOTHIAZIN COMPOUNDS FOR THE TREATMENT OF RADICAL CANALS INFECTED WITH Enterococcus faecalis.
Enterococcus faecalis. Endodontics. Photodynamic Therapy. Laser
Enterococcus faecalis is the main pathogen responsible for failures in endodontic
treatments in clinical dentistry. Its ability to penetrate dentinal canaliculi, resist to large
variations in pH, high capacity to produce biofilm, resistance genes and efflux pumps
makes it highly resistant to conventional pharmacotherapies and classical endodontic
treatments. The aim of this study was to evaluate the efficacy of in vitro photodynamic
therapy using Taylor blue in nanocontractions of 332 ηg/mL and 689.9 ηg/mL
associated with red laser coupled to optical fiber (λ660 ηm) in pre-irradiation times of
5 and 10 minutes on the entire biofilm of E. faecalis in root canals. All experiments
were performed in triplicate and the results used the ANOVA statistical test with
Tukey's multiple comparison and post-test the GraphPad Prism® 6.0 software. TFDa
was performed in two protocols 1 and 2, which showed significant reductions in
microbial load (p<0.001 and p<0.01) when compared to the positive control group. The
percentage of microbial reduction of intracanal E. faecalis whole biofilm culture in these
groups were 90% in protocol 1 and 90.3% in protocol 2. Conclusion, the findings
demonstrated that TFDa showed promise when used against E. faecalis whole biofilm
in the root canal, making it a viable, therapeutic alternative for use in dentistry because
it is efficient, can be modulated according to the need for treatment, and there is no
evidence of induction of resistant strains of the microorganism.