Banca de DEFESA: WALERIA BORGES DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : WALERIA BORGES DA SILVA
DATA : 22/02/2019
HORA: 14:00
LOCAL: Auditório EMEVZ-UFBA
TÍTULO:

Antigenic comparison between Sarcocystis neurona and Sarcocystis falcatula-like. isolated in vitro in Bahia.

PALAVRAS-CHAVES:

opossum, western blot, immunofluorescence

PÁGINAS: 44
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
ESPECIALIDADE: Doenças Parasitárias de Animais
RESUMO:

Sarcocystis neurona is a coccidian parasite, considered the causative agente of equine protozoal mieloencephalitis (EPM). Opossums of the genus Didelphis are known as definitive hosts of S. neurona. Four species of opossums (Didelphis sp.) are found in Brazil. The aim of this study was to compare a national isolate of Sarcocystis sp. with a strain of S. neurona for potential use in serological tests. 217 equine serum samples were tested by indirect Immunofluorescence technique (IFAT) with merozoites produced in the cell culture of the two parasites. Preliminary results at IFAT revealed that frequency of exposing animals to S. neurona was 7.83% and Sarcocystis sp. 8.76%. Eight seropositive animals by IFAT against S. neurona were tested by Western blot with non-reduced S. neurona antigen. Proteins with molecular weight of 18 kDA was identified. Preliminary results, with positive reaction in both strains, allow two possible conclusions, that the animals have been exposed to both parasites tested, or horses was exposed to one of the parasites, but cross-reactivity was seen in the IFAT. In case the occurrence of cross-reactivity is verified, there will be support for the use of national isolates in the IFAT at EPM diagnosis.

MEMBROS DA BANCA:
Presidente - 1085375 - LUIS FERNANDO PITA GONDIM
Interno - 1561789 - RICARDO WAGNER DIAS PORTELA
Externo ao Programa - 2339562 - FLAVIANE ALVES DE PINHO
Externo à Instituição - WENDELL MARCELO DE SOUZA PERINOTTO