Banca de DEFESA: LAIS PEREIRA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LAIS PEREIRA SILVA
DATA : 25/02/2019
HORA: 13:30
LOCAL: Congregação EMEVZ-UFBA
TÍTULO:

Validation of a bioanalytical method to evaluate the influence of ivermectin on the pharmacokinetics (pk) of carboplatin - preclinical test

PALAVRAS-CHAVES:

liquid chromatography, chemotherapy, rats

PÁGINAS: 77
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Patologia Animal
ESPECIALIDADE: Patologia Clínica Animal
RESUMO:

The number of studies aimed at elucidating the new therapeutic guidelines is increasingly increasing for mammary neoplasms in bitches. In this context, a study was carried out to validate the carboplatin analysis method in the plasma of rats and women to evaluate the influence of proliferation and cyclophosphamide on the pharmacology of carboplatin in healthy wistars - preclinical medical test. We studied the 24 wistars, divided into three experimental groups, Group 1 consisting of animals with carboplatin alone (n = 8), Group 2 animals treated with carboplatin preceded by applications of Ivermectin and Group 3 animals treated with carboplatin associated with cyclophosphamide in metronomic regimen (n = 8). It was collected after general anesthesia of all animals at the same times 0, 5, 15, 30, 45, 60, 120 and 240 minutes after administration of carboplatin, where the animals were euthanized and referred to the pathology sector. The following were collected: kidneys, intestine, fragments of liver and lung. Blood samples were directed to the pharmacokinetic analysis using the noncompartmental model HPLC-UV. The method had full validation in bitches, as this study is a pilot for evaluation of pharmacokinetics in female dogs with mammary carcinomas. The same method was linear and reproducible for plasma analyzes of Wistars rats (pilot study). The data were a modest trend to increase the shelf-life of the ivermectin group when it became a pilot group (carboplatin), which may also be noted in reducing clearance of the drug in the same group. With the result of the histopathological, we did not detect only cases of discrete circulatory and degenerative alterations in the renal and hepatic parenchyma.

MEMBROS DA BANCA:
Presidente - 1489010 - ALESSANDRA ESTRELA DA SILVA LIMA
Interno - 1283878 - STELLA MARIA BARROUIN MELO
Externo ao Programa - 1012812 - FRANCINE JOHANSSON AZEREDO
Externo à Instituição - KARINE ARAUJO DAMASCENO