Banca de DEFESA: ANA CLAUDIA SANTOS RAPOSO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANA CLAUDIA SANTOS RAPOSO
DATA : 19/02/2019
HORA: 08:00
LOCAL: Congregação
TÍTULO:

QUALITATIVE EVALUATION OF TETRAPHOR TEARS

PALAVRAS-CHAVES:

Biochemical composition; Comparative analysis; Glicoproteomic; Proteomic Ocular surface; Vertebrates.

PÁGINAS: 245
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
ESPECIALIDADE: Clínica Cirúrgica Animal
RESUMO:

Tear is a complex fluid involved in nourishment, stability, protection and removal of foreign bodies from ocular surface. There are studies regarding its components and dynamics for mammalian species; however, the knowledge about this fluid in other species is limited. The evolutive process for adaptation and environmental influences may inducted changes on vertebrate tears. In addition, qualitative and quantitative differences can occur, even in animals phylogenetically related. Therefore, the objective of this research was to perform qualitative-quantitative evaluation of tetrapod tears (reptiles, birds and mammals), using unpublished methodologies for wild animals. The protein profile and biochemical composition of the tear of reptiles, birds and mammals, present in different ecological niches, were evaluated comparatively with blood serum and  human tear, and it was obtained that the tear of the animals contained the same compounds present in the tear human and blood serum in different proportions. Together with this finding, it was attributed that the phylogenetic proximity, diet and environment, the latter in a significant way, influence on the tear components. From these results, some species were selected because of their notorious differences in the electrophoretic profile for the proteomic investigation of the tear, as hawk (Rupornis magnirostris), caiman (Caiman latirostris) and sea turtle (Caretta caretta). The proteins found, as well as their ontogenic characteristics, have demonstrated that the tear composition can be derived from the environmental conditions and lifestyles of the animals, being able to adapt to the complex adaptation of the species. Besides, even before the most primitive metabolic processes, there is an maintenance in the stability mechanisms of tears. So, the tear fluid pervades what is described for mammals and is a fluid with high adaptation power. It has roles in maintaining ocular surface homeostasis and consequently visual function.

MEMBROS DA BANCA:
Presidente - 1675087 - ARIANNE PONTES ORIA
Interno - 1213625 - CARLOS ROBERTO FRANKE
Externo ao Programa - 2421295 - RODRIGO BARBAN ZUCOLOTO
Externo ao Programa - 2415395 - THIAGO LUIZ DE PAULA CASTRO
Externo ao Programa - 1676932 - VITOR HUGO MOREAU DA CUNHA