Banca de DEFESA: MONICA MADRIGAL VALVERDE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MONICA MADRIGAL VALVERDE
DATA : 04/02/2019
HORA: 09:00
LOCAL: Escola de Medicina Veterinária e Zootecnia da UFBA
TÍTULO:

Uretral Sperm criopreservarion on Domestic and Wild Feline, sperm parameters and corporal and testicular biometry correlation.

PALAVRAS-CHAVES:

α2 adrenerc agonist; Sperm quality; testicular volume; computer-assisted sperm analysis; sperm subpopulation, flow citometry.

PÁGINAS: 172
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
ESPECIALIDADE: Ginecologia e Andrologia Animal
RESUMO:

The information obtained on the biology and reproductive biotechnology of the domestic cat allows a correlation and application in feline wild species. The study of the physical characteristics and their association with reproductive parameters allows to increase the selection criteria of individuals for reproduction of endangered species. Semen cryopreservation is a fundamental component for assisted reproduction programs in wild felids. The objective of this study was to evaluate the association between the biometric measurements and the before and after freezing morphokinetic characteristics of feline domestic and wild species sperm , obtained by uretral cateterization using two different drugs and processed with three seminal extenders . For this study, 13 feline males (Felis catus), selected and separated into groups, according to the drug tested (Experiment 1). In the first experimental group (GD1), the combination of medetomidine hydrochloride (0.1 mg / kg) and ketamine (5 mg / kg) was used and in the second group (GD2) the combination of dexmedetomidine hydrochloride (1 mg / kg ) and ketamine (5 mg / kg). After the required anesthetic level was reached, the animals were submitted to probing with urethral catheter, and the semen was collected and transported to the laboratory for analysis. We evaluated the semen quality characteristics of fresh and frozen-thawed sperm with conventional microscopy: volume, total motility and progressive motility, percentage of spermatozoa with membrane functional integrity (osmotic shock) and membrane structural integrity (eosin stain), sperm concentration and sperm morfology. There was no difference in sperm parameters (P> 0.05) into groups GD1 and GD2. The samples were cryopreserved in three groups of cryoprotectants (Experiment 2), GC1 with 6% glycerol ( GLI ), GC2 with 3% dimethylacetamide ( DMA ) GC3 with 3% dimethylformamide ( DMF ). For frozen-thawed semen, kinetic parameters in computerized analysis equipment (CASA) and fluorescence in flow cytometry were also evaluated, when there were also no differences (P> 0.05) for the different parameters evaluated between the groups studied. The study of spermatic subpopulations identified four subpopulations with independent behaviors defined between them. After the semen measurements and collection, the animals were submitted to orchidectomy. In the animals, corporal and testicular measurements were performed: head length, head circumference, head +body length, thoracic diameters, tail length and total length, width, length, thickness of each testicle, and body weight (Experiment 3). In addition, the testes of the animals were measured with ultrasonography. Post-orchiectomy testicles were also measured and weighed. The estimated testicular parameters were testicular area, testicular volume, testicular weight and gonadosomatic index. We compared the methods of testicular measurements and estimated linear Pearson correlations between body biometry, testicular and sperm parameters. There were no differences between the measurements obtained with the two methods of measuring the testicles (P> 0.05). Positive correlations (P <0.05) were observed between total length and testicular parameters, as well as between sperm concentration and testicular parameters. For the study with the wild felids, a Leopardus wiedii, a Puma yaguaroundi and an Panthera ounce. The animals were submitted to chemical ejaculation with the combination of medetomidine hydrochloride (0.1 mg / kg) and ketamine (5 mg / kg) IM. Seminal samples collected by urethral catheterization were frizing with the same cryoprotectants and concentrations of Experiment 2. The evaluations of the in fresh and post-thaw semen were the same as described for Experiment 1 and 2, which comparison between the different groups resulted in Experiment 4 For the latter study, no differences were found for the extender used (P> 0.05). It was concluded that there are no differences in sperm quality in fresh and post-thawing. However, testicular biometry exerts an important influence on the sperm concentration of the breeders, being an adequate parameter to be considered in felid breeding.

MEMBROS DA BANCA:
Presidente - 1085368 - ANTONIO DE LISBOA RIBEIRO FILHO
Interno - 285741 - MARIA CONSUELO CARIBE AYRES
Interno - 2011095 - VIVIAN FERNANDA BARBOSA
Externo à Instituição - ANA KARINA DA SILVA CAVALCANTE - UFRB