Banca de DEFESA: RAFAEL TEIXEIRA DA SILVA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : RAFAEL TEIXEIRA DA SILVA
DATE: 14/07/2022
TIME: 14:00
LOCAL: meet.google.com/wgx-ccpn-ywz
TITLE:
Effects of high-fat diet consumption during pregnancy, lactation and post-weaning periods on the bone structure of adult rats
KEY WORDS:
High fat diet, Bone structure, Metabolic programming, Rats.
PAGES: 87
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUBÁREA: Desnutrição e Desenvolvimento Fisiológico
SUMMARY:
Introduction: Recent studies suggest that consumption of diets high in fat promotes metabolic changes, and these impacts also affect the bone structure, however little is said about the relationship from consumption in the gestation and lactation phases. Objective: To evaluate the effects of high-fat diet consumption during pregnancy, lactation and post-weaning periods on the bone structure of adult rats. Methodology: The study was divided into two stages. In the first, a systematic literature review was developed by searching the PubMed/Medline, Embase, Scopus and Web of Science databases. The inclusion criteria of the review were: use of hyperlipidic diets, adult Wistar rats, and bone structure analysis. In the second stage of the study, an experimental study was conducted in rats. The animals were divided into four subgroups: Control/Control (CC, n=6) – the mother consumed a control diet and the offspring maintained consumption after weaning; Control/Hyperlipid (CH, n=5) – the mother consumed the control diet and the offspring consumed the hyperlipidic diet after weaning; High Fat/Control (HC, n=9) – the mother consumed the high fat diet and the offspring the control after weaning; Hyperlipidic/Hyperlipidic (HH, n=8) – the mother consumed the high-fat diet and the offspring maintained consumption after weaning. Upon completing 90 days of life, the animals were euthanized and desiccated for organ collection. The weight and length of the animals were analyzed, and later the Lee index was calculated. The amount of retroperitoneal adipose tissue and the dimensions of the femur (weight, distance between the epiphyses, distance between the trochanters and width of the midpoint of the diaphysis) and of the 4th lumbar vertebra (weight and length) were analyzed. Statistical analysis was performed using the GraphPadPrism 5.0 program, and the Kolmogorov-Smirnov normality test was used. For the parametric data, ANOVA one way, followed by Tukey or Bonferroni was used. For non-parametric data, the Mann-Whitney U test was used, followed by Kruskal-Wallis or Dunns. The study was approved by the Ethics Committee on Animal Experimentation of the Faculty of Veterinary Medicine and Animal Science at UFBA under protocol 04/2019. Results: The Lee index showed no statistical difference in the mean values between the groups. The amount of absolute and relative retroperitoneal adipose tissue was statistically higher in the HH and CH groups compared to the CC group. Femur weight was statistically lower in the HH and CH groups when compared to the CC group. The 4th lumbar vertebra had a statistically lower weight in the HH group compared to the CC group. The distance between the epiphyses and the width of the midpoint of the diaphysis were statistically smaller in the HH and CH groups compared to the CC group. The distance between the trochanters was statistically smaller in the HH group when compared to the CC group. The length of the 4th lumbar vertebra showed no statistical difference between the groups. Conclusion: The results of this study suggest that the high-fat diet when consumed during pregnancy, lactation and postweaning can harm the bone structure of offspring
BANKING MEMBERS:
Presidente - 053.537.227-28 - CARLOS ALBERTO SOARES DA COSTA - UERJ
Externa à Instituição - ALINE DAVILA PEREIRA
Externa à Instituição - RHOWENA JANE BARBOSA DE MATOS