Banca de DEFESA: LUCIMEIRE SANTANA DOS SANTOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LUCIMEIRE SANTANA DOS SANTOS
DATA : 29/09/2020
HORA: 08:30
LOCAL: https://conferenciaweb.rnp.br/webconf/jairza-maria-barreto-medeiros
TÍTULO:

High-fat diet during the perinatal and / or post-weaning period: effects on the genes expression related to the control of food intake and inflammation in adult rats 

PALAVRAS-CHAVES:

Food intake, high-fat diet, gene expression, pregnancy, inflammation, lactation, rats.

PÁGINAS: 127
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Nutrition during the period of development can have lasting repercussions for the health of the being in training. In this way, a nutritional manipulation of periods is a relevant study model for understanding the modulation of phenotypic plasticity and its repercussions. Among the repercussions are molecular changes, more specifically in gene expression. The present study aimed to investigate the effect of exposure to a high-fat diet during intrauterine life and lactation associated or not with the maintenance of food intake of high-fat diet after weaning on the genes expression related to the control of food intake and inflammation in rats adults. This study was approved by the Animal Use Ethics Committee of the Faculty of Dentistry of the Federal University of Bahia (nº 02/13). Animal handling and care followed the recommendations of the Brazilian Society of Laboratory Animal Science (SBCAL) and ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments). The animals were kept in the Experimental Nutrition Laboratory of UFBA (LABNEX), under a temperature of 23 ± 2 ° C and a light / dark cycle of 12 hours. Wistar rats were divided into four subgroups: Control / Control (CC, n = 5) - descendants of rats fed the control diet and fed the same diet after weaning; Control / High-fat diet (CH, n = 5) - offspring of rats fed a control diet and fed a high-fat diet after weaning; High-fat diet / Control (HC, n = 5) - offspring of rats fed a high-fat diet in the perinatal period and fed a control diet in the post-weaning period; High-fat diet / High-fat diet (HH, n = 5) - offspring fed a high-fat diet in the perinatal period and after weaning. After euthanasia and dissection of the white adipose tissue, hypothalamus and nucleus accumbens, analyzes of the drd1, drd2, pomc, npy, tnf-α and nf-kb genes expression using the real-time PCR technique. The CH group showed greater expression of drd1 and drd2, the HC group showed greater expression of drd2 and the HH group showed greater expression of drd1. The HC group showed greater pomc expression and the HH group showed greater pomc and npy expression. The CH and HH groups showed higher expression of nf-kb and tnf - α in omental and retroperitoneal white adipose tissue and the HC and HH groups showed higher nf-kb expression in hypothalamus. Thus, the high-fat diet altered the genes expression related to hedonic and homeostatic control of food intake and the expression of inflammatory genes, more intensely in animals exposed to a high-fat diet in the critical stages of development and who continued to intake same diet after weaning.

MEMBROS DA BANCA:
Presidente - 1066101 - JAIRZA MARIA BARRETO MEDEIROS
Interno - 3112241 - MARIA ESTER PEREIRA DA CONCEICAO MACHADO
Interno - 1194475 - MONICA LEILA PORTELA DE SANTANA
Externo ao Programa - 1490663 - RICARDO DAVID COUTO
Externo à Instituição - CRISTIANO MENDES DA SILVA - UNIFESP