Banca de DEFESA: CLARISSA CUNHA SANTANA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CLARISSA CUNHA SANTANA
DATA : 12/11/2021
HORA: 09:00
LOCAL: IGM Sala Virtual 5
TÍTULO:

Biosignature associated with susceptibility to tuberculosis-human immunodeficiency virus (TB-HIV) co-infection: 

evaluation of pathways related to cell death, innate immune response and T lymphocyte activation.

PALAVRAS-CHAVES:

Tuberculosis, TB-HIV co-infection, cell death, lymphocyte profile, differentially expressed genes.

PÁGINAS: 203
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

ABSTRACT
INTRODUCTION. Tuberculosis has a high incidence and mortality in several countries around the world, even with the vaccine available. Within its spectrum of presentations, we observe from infected individuals without the disease, a state known as latent tuberculosis, to those with the most severe cases, such as those associated with chronic infections, often with strains resistant to multiple drugs used in the treatment, or co-infected with HIV. In an attempt to combat the disease, a framework of immunological factors is present, however, the mycobacterium presents efficient mechanisms for evading the immune response, such as cell death inhibition, lymphocyte response modulation and escape to the extracellular environment. METHODOLOGY. We evaluated host genetic diversity in pathways related to cell death, T lymphocyte subsets and T lymphocyte gene expression profile in TB and HIV mono-infection and TB-HIV co-infected individuals to identify a signature at the transcriptional level and cell to be used as a basis for prospecting new targets for distinguishing individuals with active tuberculosis and TB-HIV co-infected. RESULTS. Our results point to differences in the pattern of cell activation in LTBI, TB and TB-HIV, in part determined by the inverse trend of the presence of HLA-DR+PD-1+ T cells in relation to CD95+. In TB-HIV, a greater presence of central and effector memory cells was observed, and Tcm CD8+HLA-DR+ cells demonstrated to be able to discriminate co-infected individuals from the other clinical groups investigated. In coinfected patients, there is expansion of the central memory T cell compartment and, in parallel, it is verified that the AK5, FAS/CD95 and IL17A genes were able to discriminate the evaluated clinical groups. Finally, we obtained several records in genetic databases regarding the variability in molecules of cell death pathways that could act as promising targets for future interventions. CONCLUSIONS. All approaches studied indicate potential biomarkers to be explored to distinguish clinical groups of interest.

MEMBROS DA BANCA:
Externo à Instituição - KIYOSHI FERREIRA FUKUTANI
Externa à Instituição - LEA CRISTINA DE CARVALHO CASTELLUCCI - UFBA
Interno - 249.563.328-58 - LEONARDO PAIVA FARIAS - USP
Externa à Instituição - LUANA LEANDRO GOIS - EBMSP
Presidente - 512.852.405-10 - THEOLIS COSTA BARBOSA - UFBA