Banca de DEFESA: MICHAEL NASCIMENTO MACEDO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MICHAEL NASCIMENTO MACEDO
DATA : 13/07/2020
HORA: 14:00
LOCAL: Webconferência
TÍTULO:

Clinical and immunological aspects and profile of group 2 innate lymphoid cells (ILC2) of individuals with human schistosomiasis mansoni.

PALAVRAS-CHAVES:

 Schistosomiasis mansoni, Group 2 innate lymphoid cells, CRTH2, cytokines, TSLP, IL-13, fibrosis.

PÁGINAS: 143
GRANDE ÁREA: Ciências Biológicas
ÁREA: Imunologia
RESUMO:

INTRODUCTION: Schistosomiasis is a disease caused by parasites of Schistosoma genus. Five different species cause human infection, among them the Schistosoma mansoni, responsible for schistosomiasis in Brazil. This disease is distributed by 78 countries affecting about 250 million people. The acute phase of the disease is characterized by Th1 cytokine production, while in the chronic phase, there is a predominance of the type 2 cytokines, which at long term, is more related to damage instead of the curative response. A group of recently discovered cells, the type 2 innate lymphoid cells (ILC2), has been described in the context of diseases with type 2 responses, as in helminthiasis. Recent data shows that ILC2 are crucial as a source of Th2 cytokines, as IL-13, contributing to the main harmful events. Nevertheless, the role of ILC2 in human schistosomiasis is still unclear. AIM: The aim of this study was to characterize the circulating type 2 innate lymphoid cells (ILC2) in Schistosoma mansoni infected individuals. MATERIAL AND METHODS: S. mansoni infected individuals were recruited from an endemic area and the health control individuals were recruited from nonendemic area. The assessment of serum cytokines was performed after the blood was collected and serum separation, so the dosage of cytokines Th1, Th2 and Th17 was performed by ELISA and CBA. RESULTS: The present data shows that S. mansoni infected individuals present high levels of TSLP and IL-13 cytokines, and that the schistosomiasis treatment decrease these levels. Moreover, this study also shows that the participants have increased serum concentrations of the hepatic enzymes TGO, TGP, γGT e ALP. Nevertheless, Ritis coefficient, which assess the presence of chronic lesions, showed that 98,5% of the participants may present chronic lesions, likely with fibrosis or hepatic cirrhosis. Although there is no difference in the frequency of ILC2 from infected individuals and healthy controls, the individuals with schistosomiasis present more expression of CRTH2 receptor. In addition, sorting isolated ILC2 from infected individuals, also produce more IL-13 as compared to healthy controls. CONCLUSIONS: In this study, we demonstrated that schistosomiasis treatment decrease the concentrations of TSLP and IL-13 cytokines, involved in maintenance and homeostasis processes. The results also indicate that ILC2 are a font of IL-13, once infected individuals show high serum levels, and this also can be demonstrated by ILC2 in vitro.               

MEMBROS DA BANCA:
Externo ao Programa - 2138054 - ALEX JOSE LEITE TORRES
Interno - 249.563.328-58 - LEONARDO PAIVA FARIAS - USP
Externo à Instituição - LUCAS PEDREIRA DE CARVALHO - Fiocruz-Ba
Externo ao Programa - 2584365 - LUCIANA SANTOS CARDOSO
Presidente - 804.709.555-20 - RICARDO RICCIO OLIVEIRA - UFBA