Banca de DEFESA: DANIELLE DEVEQUI GOMES NUNES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANIELLE DEVEQUI GOMES NUNES
DATA : 04/07/2019
HORA: 09:00
LOCAL: Instituto Gonçalo Moniz
TÍTULO:

Chemical composition and antimicrobial and leishmanicidal biological activity of propolis extracts obtained by the conventional method or by supercritical extraction

PALAVRAS-CHAVES:

Leishmania, propolis, bacterial activity, conventional extraction, supercritical extraction, natural products

PÁGINAS: 84
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
ESPECIALIDADE: Protozoologia Parasitária Humana
RESUMO:

Propolis is a natural product with several biological activities tested and its extracts obtained by various techniques have been used in the food, pharmaceutical and cosmetic industries. Variations in the chemical composition are directly associated to the type and geographic origin of the propolis, consequently, interferes in the biological activity of the propolis. The aimed of this work was to characterize the chemical and biological activity of samples of propolis extract: red, green and brown, collected in different regions of the State of Bahia, Brazil, obtained by the conventional method (ethanolic extraction) or the supercritical extraction technique. First, we identified the physical-chemical profile of the raw samples, as for humidity content, water activity, total ash content, protein, lipids and fibers. The extracts of propolis obtained by the ethanolic and supercritical extraction were evaluated for the content of phenolic compounds, flavonoids, antioxidant activity (DPPH), catechin, ferulic acid and luteolin. The results demonstrated that ethanolic extracts, especially those obtained from red propolis, have a higher selectivity for antioxidant compounds, as well as a better antimicrobial activity against two strains of bacteria (Staphylococcus aureus and Escherichia coli), when compared to the extracts obtained by supercritical extraction, indicating that there was no significant efficiency of the parameters evaluated in this condition. Finally, we compared the leishmanicidal action of ethanolic extracts obtained from green and red propolis on the in vitro infection of macrophages by Leishmania braziliensis, the main etiological agent of the cutaneous clinical form of the disease. In this context, red propolis had a greater potential for leishmanicidal effect. The results confirm the influence of the raw material type as well as the extraction methods regarding the composition and biological activity of the different extracts of propolis, including their use as antimicrobial and leishmanicidal agents. The present study contributes with new perspectives in order to enable technological applications of propolis and its derivatives in the natural products industry.

MEMBROS DA BANCA:
Interno - 249.563.328-58 - LEONARDO PAIVA FARIAS - USP
Interno - 028.288.917-55 - VALÉRIA DE MATOS BORGES - UFRJ
Externo ao Programa - 1561789 - RICARDO WAGNER DIAS PORTELA
Externo à Instituição - DIOGO RODRIGO DE MAGALHÃES MOREIRA - Fiocruz-Ba