Banca de DEFESA: JOILSON XAVIER DOS SANTOS JUNIOR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOILSON XAVIER DOS SANTOS JUNIOR
DATA : 25/02/2019
HORA: 14:00
LOCAL: Instituto Gonçalo Moniz
TÍTULO:

GENOMIC AND EPIDEMIOLOGICAL EVALUATION OF THE CHIKUNGUNYA VIRUS CIRCULATING IN RIO DE JANEIRO

PALAVRAS-CHAVES:

chikungunya virus, Rio de Janeiro, genomic epidemiology, MinION, sequencing.

PÁGINAS: 123
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Molecular e de Microorganismos
RESUMO:

Chikungunya fever is a self-limited disease caused by chikungunya virus (CHIKV), which is transmitted by the bite of infected hematophagous mosquitoes of the genus Aedes. Most infected individuals present symptoms within 4 to 7 days, such as fever, maculopapular rash, polyarthralgia and myalgia. Cases of chikungunya in Brazil are recorded since the virus was first identified in the country in 2014. In 2018, Brazil reported 87,687 probable cases of the disease, of which 52,966 cases (60.4%) occurred in the Southeast region only. The state of Rio de Janeiro experienced a major epidemic caused by CHIKV in 2016, reporting 18,516 probable cases. The emergence of CHIKV has raised concern due to the rapid spread of the virus into new geographic areas and the clinical features associated with the infection. Thus, the objective of this study was to investigate the genomic epidemiology of chikungunya virus circulating in the state of Rio de Janeiro. We used the nanopore sequencing method that allows the generation of large amounts of data in a few hours. We successfully generated 11 CHIKV genomic sequences from serum samples from symptomatic patients residing mostly (n = 6) in the city of Rio de Janeiro. The remaining five samples were from patients residing in four neighbouring municipalities. Phylogenetic analyses, using the molecular clock approach, revealed that the strains circulating in Rio de Janeiro belonged to the ECSA African genotype and this genotype was likely introduced in the state around July 2014. This means that the virus circulated unnoticed for 16 months before the first official reports of autochthonous transmission in the state of Rio de Janeiro. Both epidemiological and phylogenetic data suggest that strains from ECSA lineage were introduced into Rio de Janeiro from CHIKV dispersion events from Northeast region of Brazil to others Brazilian regions. 

MEMBROS DA BANCA:
Presidente - 576.261.896-04 - LUIZ CARLOS JUNIOR ALCANTARA - Fiocruz - RJ
Externo ao Programa - 2766939 - JOANA PAIXAO MONTEIRO CUNHA
Externo à Instituição - VASCO ARISTON DE CARVALHO AZEVEDO - UFMG
Externo à Instituição - ISADORA CRISTINA DE SIQUEIRA - Fiocruz-Ba