Banca de DEFESA: ELLEN DOS REIS PIMENTEL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ELLEN DOS REIS PIMENTEL
DATE: 05/10/2022
TIME: 09:00
LOCAL: IGM Sala Virtual 5
TITLE:

Assessment of the adaptive immune response against CHIKV in people living with HIV/AIDS who have been infected by the arbovírus.

KEY WORDS:

HIV-1, Chikungunya fever, Immunoactivation, Memory

PAGES: 46
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

ABSTRACT
INTRODUCTION: HIV is a slowly progressing infection virus that, if left untreated, generates a state of immunosuppression that can alter the immune response profile of protective memory to subsequent infections by other viruses. In healthy individuals, CHIKV infection has high morbidity and the development of a chronic condition of arthralgia and arthritis that can last more than a year in more than 50% of those infected. In a context of co-circulation of HIV-1 and other infections, it is essential to understand how the secondary infection by arboviruses evolves in people living with HIV/AIDS (PLWHA) in treatment with athyretrovirals and if these individuals are able to produce a response protective and lasting immune system to these microorganisms. OBJECTIVE: Thus, the objective of this study was to evaluate the qualitative-quantitative profile of the memory cellular immune response of people living with HIV/AIDS with a past history of CHIKV infection treated at the STD/HIV/AIDS Reference Center in Feira de Santana, Bahia. MATERIALS AND METHODS: A total of 634 PLWHA were recruited to participate in a study of seroprevalence of previous infection with the CHIKV virus through the detection of IgG anti-CHIKV antibodies. Of these, 46 individuals with positive anti-CHIKV IgG and 67 individuals with negative anti-CHIKV IgG were invited to participate in a recollection, matched by clinical and sociodemographic data. From this, 10mL of blood was collected from selected individuals and submitted to in vitro stimulation with 1000 PFU of CHIKV. After 48h, the supernatant was used to measure the following soluble markers: CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CXCL8/IL-8, CXCL10/IP-10, EGF, Eotaxin, G -CSF, GM-CSF, IFN-α2, IFN-γ, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-1RA, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, TNF-α, TNF-β, VEGF, using Luminex technology. RESULTS: Significantly lower concentrations of EGF and higher concentrations of GM-CSF, IL-8, IP-10, MIP-1α, MIP-1β, IFN-α2, IFN-γ, IL-1RA, IL-1α, and IL-2 were observed in PLWHA with positive serology for CHIKV when compared to the PLWHA group with negative serology for CHIKV. Then, we evaluated if the significantly modulated cytokine quantifications in the group with positive serology for CHIKV were impacted by HIV-1 viral load levels, CD4+, CD8+, CD4+/CD8+ and CD45+ T cells at the time of collection. It was observed that the level of CD4+ T cells showed a significant positive association with IFN-γ,
IL1-RA, IL-2, the level of CD8+ T cells showed a significant positive association with EGF, the CD4+/CD8+ T cell ratio showed a positive significant association with IP-10, IFN-α2, IFN-γ and the level of CD45+ T cells showed a significant positive association with IL-1RA. CONCLUSIONS: Thus, we conclude that PLWHA under antiretroviral treatment are capable of developing a protective and long-lasting Th1-type cellular immune response, characterized mainly by IFN-γ, IL-2, IFN-α2 and IP-10, EGF and IL1-Rα. However, these cytokines are directly impacted by the recovery stage of leukocyte levels.

BANKING MEMBERS:
Presidente - 2236977 - ANTONIO RICARDO KHOURI CUNHA
Externa ao Programa - 2893243 - LUANA LEANDRO GOIS
Externo à Instituição - LUCAS PEDREIRA DE CARVALHO - Fiocruz-Ba