Banca de DEFESA: LAURA MARIA ANDRADE SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LAURA MARIA ANDRADE SILVA
DATA : 19/06/2020
HORA: 09:00
LOCAL: De forma remota (online)
TÍTULO:

IMMUNE-HISTOCHEMICAL STUDY OF ADHESION MOLECULES AND CYTOKERATINS IN THE DEVELOPING HUMAN HAIR FOLLICLE

PALAVRAS-CHAVES:

 Hair follicle. Fetal development. Immunohistochemistry. Adhesion molecules 

PÁGINAS: 110
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
RESUMO:

Introduction: Although much knowledge has been generated over the years about the human hair follicle and the cellular structures that comprise it, little has been described about the role of adhesion molecules and cytokeratins in their development. Objective: Identify the classes of the main adhesion and cell maturation molecules involved in the developmental stages of the developing human hair follicle. Materials and methods: Through case series study, by immunohistochemistry, we evaluated the expression of the molecules e-cadherin, p-cadherin, beta 1 Integrin, beta catenin, CD34 and cytokeratins (CK)1,10,13,14,16 and 20 in development of fetal human hair follicle at 149 skin samples from 35 embryos and fetuses of gestational ages ranging from 4 to 25 weeks. Results: CK14 was the first to be expressed in hair follicles from embryos at the 9th gestational week, followed by the expression of CK1, CK10, CK13 and CK16 at 12 gestational weeks. Subsequently, CK20 is expressed in fetuses at 14 weeks of gestation. Beta 1 integrin expressed in 100 %, e-cadherin expressed in 65,4 % and p- cadherin expressed in 76,9 % of the analyzed specimens. Beta catenin did not show immunoexpression in the cases analyzed at any concentration evaluated. It was positive at the control for tonsils and adult scalp. CD 34 immunostaining was present at 28.6% of the analyzed cases. Conclusions: About molecules studied, beta 1 integrin is the main adhesion molecule involved in the early stages of fetal follicular development. Cadherins pcadherin and e-cadherin are molecules necessary for the processes of orchestration and signaling of the initial steps of follicular formation. Of cadherins, p-cadherin is the most characteristic of the human fetal hair follicle. Beta catenin is downregulated during the fetal follicular development process and CD34 is low sensitive follicular stem cell marker. Cytokeratins are good markers of fetal follicular cell maturation.

MEMBROS DA BANCA:
Externo à Instituição - FERNANDA VENTIN DE OLIVEIRA PRATES - UNEB
Externo à Instituição - IVONISE FOLLADOR - UFBA
Externo à Instituição - MARIA DE FATIMA SANTOS PAIM DE OLIVEIRA - UFBA
Externo à Instituição - MARIA ELISA ALVES ROSA
Externo ao Programa - 163.517.385-04 - PAULO ROBERTO LIMA MACHADO - UFBA