Banca de DEFESA: LAVÍNIA SILVA CONCEIÇÃO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LAVÍNIA SILVA CONCEIÇÃO
DATA : 27/03/2020
HORA: 09:00
LOCAL: Auditorio de Geoqrafia, Instituto de Geociências, UFBA
TÍTULO:

EFFICIENCY OF MICROBIAL CONSORTIUM DEGRADATOR OF ALCAN DURING THE BIORREMEDIATION OF THE MANGUEZAL SEDIMENT WITH OIL

PALAVRAS-CHAVES:

Biodegradation. n-alkanes. DNA. Bioremediation. Factorial planning

PÁGINAS: 93
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Geociências
SUBÁREA: Geologia
ESPECIALIDADE: Geoquímica
RESUMO:

Currently, oil spills are the main threat to mangroves, since their compounds are toxic and difficult to degrade. In this sense, a bioremediation experiment was set up with mangrove sediment from the São Paulo river, Bahia - Brazil, which is often affected by the oil spill. Therefore, this work sought to understand the capacity of microorganisms in this region to degrade oil in the Recôncavo basin. The design of the experiment took place through factorial planning, and included seven reactors connected to the vacuum pump, simulating the tidal regime every 6 hours. The reactors contained 400g of sterile sediment, 4g of crude oil and varying amounts of encapsulated consortium, in order to simulate the mangrove bioremediation process. The alkB gene, described as n-alkane degrader, was quantified and compared with microbial growth and the HTP / UCM ratio during three times of the experiment (4 days, 17 days and 30 days). Geochemical analyzes of nutrients (organic carbon, nitrogen and phosphorus) and HTP (total oil hydrocarbons), microbiological analyzes (enumeration of fungi and bacteria) and molecular analysis (Amplification of the gene through the technique of Real-time PCR-qPCR). The results showed that there was a positive correlation between the number of copies of alkB and the degradation of oil, and the factorial design pointed out that time influences the amount of intercropping, which showed greater degradation after the seventeenth day of the experiment.

MEMBROS DA BANCA:
Presidente - 816.415.245-53 - DANUSIA FERREIRA LIMA - UFBA
Interno - 1919894 - FABIO ALEXANDRE CHINALIA
Externo ao Programa - 957.032.685-91 - SARAH ADRIANA ROCHA SOARES - UFBA
Externo à Instituição - EDDY FRANCISCO DE OLIVEIRA - UEFS