Geographic song variation of Attila rufus (Aves: Passeriformes)
Gray-hooded Attila, variation song, acoustic adaptation, ecological selection
The vocalizations emitted by birds are classified as songs or calls, which are usually associated with mate choice, territory defense and alarms. These vocalizations play an important role on intraspecific recognition, and their variation bird vocalization can mediate pre-zygotic barrier and act in the speciation process. Most of the information regarding the vocal variation of birds comes from studies with birds that learn their songs, the oscines. Herein we evaluated the geographic variation for the two vocalizations (dawn song and day song) of Attila rufus, a tyrannid endemic to the Brazilian Atlantic Forest, that has two subspecies described based on the plumage color pattern. Therefore, our study aimed to answer the following questions: (i) Is there geographic variation in day and dawn song for Attila rufus? If so, does it present different patterns between the two vocalizations? (ii) Is Vocal variation correlated to the effects of ecological selection (vegetation and climate) and/or drift (isolation by distance)? (iii) Do Attila rufus subspecies exhibit vocal differentiation? Our discriminant analyzes results did not reveal acoustic structure in the multivariate space that corroborated the described species, agreeing with the absence of population structure previously observed in the molecular data. Only two parameters of the third note of day song exhibited a significant relationship with habitat, most specifically the temperature variation, concurring with the hypothesis of acoustic adaptation. Overall, our results indicate: (i) similar vocal variation among the subspecies, with no distinct vocal signature; (ii) habitat and geographic distance do not significantly explain the vocal variation in A. rufus; (iii) among habitat variables, only temperature influenced spectral parameters. Further analyzes of plumage variation through species distribution will help to comprehend the results found in this study.