Banca de DEFESA: JULIANA GUERREIRO CEZAR

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JULIANA GUERREIRO CEZAR
DATA : 06/08/2021
HORA: 09:00
LOCAL: Instituto de Química
TÍTULO:

SYNTHESIS AND CHARACTERIZATION OF RUTENUM AND COBALT COMPLEXES WITH CYCLIC ALIPHATIC AMINES: INTERACTION STUDIES WITH NITROGEN BASES AND DNA

PALAVRAS-CHAVES:

DNA interation, Tetraazamacrocycles, Methionine

PÁGINAS: 220
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Inorgânica
ESPECIALIDADE: Química Bio-Inorgânica
RESUMO:

Coordination compounds have fundamental importance in biological processes, since they bind and interact with molecules/biomolecules indispensable to life. In this sense, complexes containing aminoacids as binders have been synthesized, as these systems facilitate studies of the coordination of metal ions with DNA, which, in turn, indicates a potential biological application. Non-heme macrocyclic ligands are also important in chemical coordination, which is due to the presence of this type of ligand in the structures of a large number of biologically important complexes, as well as the kinetic and thermodynamic stability that these ligands confer on the complexes. NO plays key roles in several biological processes that depend on their local concentration, motivating the development of NO-releasing/capture metallo-drugs. This thesis is an investigation of the interactions of Ru and Co complexes with biomolecules, such as nitrogenous bases and DNA, aiming at the development of metal-pharmaceuticals, using as co-ligands macrocycles and nitric oxide. The unpublished complexes synthesized were: cis-[Ru(dmso)2(R-cyclen)]Cl, [Co(Met)L(mac)]n+ , cis- [Ru(Met)2(mac)]+ and [Ru(NO)(Met)(mac)]2+ . Analysis of the vibrational spectra showed the appearance of bands characteristic of the methionine and macrocyclic ligands (cyclam / cyclen/ R-cyclen), as well as allowed to assign the assignment of the monodentate coordination mode for the bond between the carboxylic group of the amino acid and the metal center. For complex nitrosyls, the identification of a characteristic band of NO in the form of NO+ is also noteworthy. The electron spectra presented bands associated with d-d and MLCT transitions and, for the Ru(II) complexes of LMCT. Electrochemical studies allowed evaluating the redox processes centered on the metallic center, with evidence of a coupled reaction with the formation of an aquacomplex, in addition to qualitatively investigating the release of methionine (or DMSO, for the complex with substituted cyclen), when submitted to electrochemical stimulation. Preliminary kinetic reactivity data confirm the aquation of chloride and DMSO in the complexes trans-[CoCl2(cyclam)]Cl and cis-[Ru(dmso)2(R-cyclen)]Cl, respectively, and confirm the entry of amino acid while the water molecule leaves the Co(III) coordination sphere. Interaction studies of chlorocomplexes with DNA were sufficient to suggest that these complexes interact with DNA. 1H NMR data, together with spectroscopic and electroanalytical analysis, confirm the proposed structures for the new complexes. Therefore, the results obtained support the formulation of the new synthesized complexes, as well as suggest the interaction of the respective chlorocomplexes with DNA.

MEMBROS DA BANCA:
Presidente - 1673526 - MARCOS MALTA DOS SANTOS
Interna - 1756798 - ELISANGELA FABIANA BOFFO
Interna - 3156252 - LUIZA AMIM MERCANTE
Interna - 1100392 - SORAIA TEIXEIRA BRANDAO
Interno - 2356272 - ARTUR JOSE SANTOS MASCARENHAS
Externo ao Programa - 2474717 - KLEBER QUEIROZ FERREIRA
Externa à Instituição - ROSENIRA SERPA DA CRUZ - UESC
Externo à Instituição - DANIEL DE LIMA PONTES - UFRN
Externo à Instituição - CARLOS DANIEL SILVA DA SILVA - IFBA