Banca de DEFESA: MESSIAS SANTOS PASSOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MESSIAS SANTOS PASSOS
DATA : 17/12/2020
HORA: 08:00
LOCAL: UNIVERSIDADE FEDERAL DA BAHIA
TÍTULO:

Bioremediation, Chemical and Biotechnological Profiling of Cadmium Resistant Filamentous Fungi

PALAVRAS-CHAVES:

BIORREMEDIATION
TOXIC METALS
CHEMICAL PROFILE
FILAMENT FUNGI
CADMIUM

PÁGINAS: 290
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

PASSOS, M. S. Biorremediação, Perfilamento Químico e Biotecnológico de Fungos Filamentosos Resistentes a cádmio. 290 p. Tese de Doutorado - Programa de Pós-Graduação em Química. Instituto de Química, Universidade Federal da Bahia, Salvador, 2020.

ABSTRACT

 

Filamentous fungi are widely disseminated in various habitats as decomposers of organic matter, among which are disease-causing species in plants and animals, including humans. On the other hand, microorganisms belonging to the Fungi Kingdom also present a set of qualities that are of extreme interest to the food and pharmaceutical industries and, more recently for employment in environmental recovery processes. In this work, the isolation of soil fungi was performed to investigate its biotechnological potential, its ability to resist toxic metal stress and its possible application in bioremediation processes, as well as the evaluation of the effect of this stress on the production of substances of special metabolism. In the isolation of the species, the technique of serial dilution of soil suspension was used, and the isolates were identified at the gender level by optical microscopy. The isolates were submitted to tests of investigation of the production of enzymes and fungicide and antibacterial agents, by development in Petri dish. They were also submitted to tolerance, resistance and bioaccumulation tests of cd (II) metal cations with analysis by flame atomic absorption spectroscopy (FAAS).Crude extracts from mycelium with organic solvent in an ultrasonic bath and from fermented broth were obtained by liquid/liquid partition with organic solvent from the species that showed greater resistance to metallic stress, both of the fungi developed in cation containing the cations and in a free medium.The extracts were studied in a mass spectrometer by electrospray ionization with an ion trap analyzer (EM-ESI-Ion Trap) for the presence or suppression of sorbcillinoid derivatives. From a total of 51 isolates, species belonging to 4 different genera were detected. In the antagonism tests, approximately half of the isolates presented a degree of growth inhibition of the microorganisms tested, with emphasis on antifungal activity.More than 70% of the isolates presented high proteolytic, amylolytic and lipolytic activity. Many isolates showed tolerance to Cd (II) at concentration 100 mg.L^-1, but only four isolates, of the genus Penicillium, showed high resistance to metallic stress developing in concentrations between 500 mg.L^-1 and 9,000 mg.L^-1.The Penicillium sp. M04 presented the highest bioaccumulation potential (in vivo) with the ability to remove 79.48 mg/g of Cd (II) from the growth medium. In the biosorption test, the isolate Penicillium sp. M04 also presented the highest Cd (II) mass values adsorbed by gram of biomass of its mycelium (inactive). The four species had their chemical profiles affected by metallic stress. The extract fractionation of the fermented broth of Penicillium sp. M01 led to the isolation of the polyketide sorbicillinoid trichodimerol. The study showed the great potential of fungi to perform bioremediation processes, because they were highly resistant and performed bioaccumulation and biosorption of Cd (II).

MEMBROS DA BANCA:
Interno - 3768242 - PAULO ROBERTO RIBEIRO DE JESUS
Interno - 1100392 - SORAIA TEIXEIRA BRANDAO
Interno - 1756798 - ELISANGELA FABIANA BOFFO
Externo ao Programa - 2053589 - EDITH CRISTINA LAIGNIER CAZEDEY
Externo ao Programa - 1477001 - REGINA MARIA GERIS DOS SANTOS
Externo à Instituição - MADSON DE GODOI PEREIRA - UNEB
Externo à Instituição - FERNANDO FAUSTINO DE OLIVEIRA - UESC-BA
Externo à Instituição - DÉBORA DE ANDRADE SANTANA - UNEB
Externo à Instituição - MARTA VALERIA ALMEIDA S. DE ANDRADE - UNEB