Banca de DEFESA: DANILO KLEBER SANTOS SALES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANILO KLEBER SANTOS SALES
DATA : 23/10/2020
HORA: 09:00
LOCAL: Ambiente Virtual RNP/UFBA
TÍTULO:

Synthesis, characterization and reactivity of ruthenium (II) indazole complex: potential anticancer agent

PALAVRAS-CHAVES:

Ruthenium(II), Indazole, Anti-cancer agent, HepG2, Metallodrug

PÁGINAS: 128
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Inorgânica
ESPECIALIDADE: Química Bio-Inorgânica
RESUMO:

Ru(II)-based complexes have been widely reported in literature as a class of compounds that have anticancer activity, due to its interaction with cellular structures, which results in the death of tumor cells. In this work, novel complex cis-[Ru^II(1H-indazole)₂(2,2-bipyridine)₂](PF₆)₂ was synthesized. Characterization by elemental analysis, spectroscopy and electrochemistry validated the formulation of complex. Density functional theory (DFT) study provided insight into the composition of molecular orbitals of the ground state in gaseous phase, and time-dependent DFT (TD-DFT) results elucidated the nature of eletronic transitions absorption in acetonitrile, indicating λ_max=445 nm is assigned to metal-ligand_bipyridine charge transfer (MLCT). The complex can act as Brønsted-Lowry acid against bases and protonated specimen are predominant in physiological. Photosubstitution reaction occurred when blue LED was irradiated in acetonitrile solution, indicating that compound is photosensitizer.  In vitro anticancer studies revealed that the complex is cytotoxic against hepatocellular carcinoma (HepG2), with the half maximal inhibitory concentration (IC₅₀) equivalent to 7.2 µmol L^-1, inducing mainly DNA fragmentation, which resulted in arrest of S phase and HepG2 cell death by apoptosis. Against healthy human pulmonary fibroblast cells (MRC-5), the compound healthy revealed non-cytotoxicity, exhibiting high selectivity. The results obtained indicated that the complex has potential to be used in live-cancer treatment, which will be supported by further studies.

MEMBROS DA BANCA:
Presidente - 2494172 - LILIAN MARIA TOSTA SIMPLICIO RODRIGUES
Externo ao Programa - 2474717 - KLEBER QUEIROZ FERREIRA
Externo à Instituição - CARLOS DANIEL SILVA DA SILVA - IFBA
Externo à Instituição - DENISE SANTOS DE SA - IFBA
Externo à Instituição - FRANCISCO ORDELEI NASCIMENTO DA SILVA - UFRN