Banca de DEFESA: MIKHAEL PEREIRA MARQUES VIVAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MIKHAEL PEREIRA MARQUES VIVAS
DATA : 13/03/2020
HORA: 14:00
LOCAL: Instituto de Química
TÍTULO:

Method development and validation by UFLC-PDA-FLD for determination of endocrine disruptors in water matrices

PALAVRAS-CHAVES:

Endocrine disruptors, method development, liquid chromatography

PÁGINAS: 76
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Analítica
ESPECIALIDADE: Análise de Traços e Química Ambiental
RESUMO:

Endocrine disruptors (ED) are species with the potential to affect endocrine system capacity at various levels, acting on steroid biosynthesis and or estrogen receptors (ERα and ERβ), resulting in inhibition, blocking or promoting endocrine system activity. Imbalance of the endocrine system directly affects the reproductive capacity of the species, reports of feminization of the population of bird, fish and mammal species can be found in the literature. In humans these substances can promote early puberty in girls, decrease the quality of semen in men and various health problems such as cancer, diabetes and obesity. In this work a chromatographic method was developed and validated for the determination of the following substances: Bisfenol A (BPA), estriol (E3), estrone (E1), 17β-estradiol (E2), 17α-ethinylestradiol (EE2), 4-n-nonylphenol (4-n-NP), 4-noctylphenol (4-n-OP), 4-t-octilfenol (4-t-OP), dimethylphthalate (DMP), diethylphthalate (DEP), butylbenzylphthalate (BBP), di-butylphthalate (DBP), di(2-ethylhexyl) phthalate (DEHP) and di-n-octyl phthalate (DnOP) in different water matrices in an acetonitrile (B) / water (A) system. Was used an ultra fast liquid chromatograph coupled to photodiode and fluorescence array detectors, column Shim-pack XR- Phenyl (100 x 2.0 mm, 2.20 μm) The running time was 16 minutes, with repeatability ranging from 4.8% (BBP) to 11.2% (E1) in the inter-day test for four days, LOD ranged from 2 μg L-¹ (E3) to 294 μg L-¹ BBP, and the linearity between 0.9236 (BPA) and 0.9977 (DEHP).

MEMBROS DA BANCA:
Presidente - 1553299 - GISELE OLIMPIO DA ROCHA
Interno - 1303987 - MARIA ELISABETE MACHADO
Interno - 1698441 - RENNAN GEOVANNY OLIVEIRA ARAUJO
Interno - 285528 - VANIA PALMEIRA CAMPOS
Externo ao Programa - 2134515 - ANA CECILIA RIZZATTI DE ALBERGARIA BARBOSA