Banca de DEFESA: BRUNA OLIVEIRA DO NASCIMENTO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNA OLIVEIRA DO NASCIMENTO
DATA : 18/06/2019
HORA: 14:30
LOCAL: Instituto de Química
TÍTULO:

PHYTOCHEMICAL STUDY AND  BIOLOGICAL ACTIVITIES EVALUATION OF THE EXTRACTS AND CONSTITUENTS OF Macrolobium rigidum (R.S Cowan)

PALAVRAS-CHAVES:

Macrolobium rigidum, antioxidant activity, antimicrobial, anticholinesterase, glycosylated coumarin.

PÁGINAS: 68
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
RESUMO:

This work describes the first phytochemical and biological study of aerial parts of Macrolobium rigidum. The extracts obtained by partition of the crude methanolic extract of the leaves and the stem of M. rigidumwere submitted to in vitrobiological activity tests: cytotoxic against A. salina microcrustacean, evaluation of the antioxidant activity measured by the DPPH radical scavenging assay method , anticholinesterase, by the microplate method, evaluating the inhibition of the acetylcholinesterase enzyme, and antimicrobial against the bacteria S. aureus, P. aeruginosa,Salmonellaand E. coli, by means of determination of the minimum inhibitory concentration (MIC) detected with the aid of the developer resazurin. The determination of the total phenolic content by the Folin-Ciocalteu method was also performed. None of the extracts presented toxicity, in contrast to the DPPH radical, the extracts in EtOAc presented activities in concentration of 10μg.mL-1, the extracts in EtOAc and in BuOH of the stem presented minimum inhibitory concentration against Salmonellaof ≤125μg.mL-1, in comparison to P. aeruginosa, leaf extracts had lower MIC values. The contents of total phenolic compounds in extracts of M. rigidumranged from 46.438 ± 0.006 to 446.668 ± 0.018 mg EGA/ g of extract. The triterpenes friedelin and taraxerol, the steroid mixture composed of β-sitosterol, capesterol and stigmasterol, the flavonoids, among them apiginine and an unusual glycosylated coumarin, were all isolated by means of chromatographic methods, all identified by analysis of the data of ¹H, ¹³C, HSQC and HMBC NMR spectra, IR region and UV-vis region spectra and mass spectrum.

 

MEMBROS DA BANCA:
Presidente - 1068075 - JORGE MAURICIO DAVID
Interno - 1215450 - SILVIO DO DESTERRO CUNHA
Interno - 1148522 - ELIANE DE OLIVEIRA SILVA
Externo à Instituição - LOURINALDA LUIZA DANTAS DA SILVA SELVA DE OLIVEIRA - UFRPE
Externo à Instituição - CLAYTON QUEIROZ ALVES