Banca de DEFESA: THAIS LUZ DE SOUZA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : THAIS LUZ DE SOUZA
DATE: 19/04/2024
TIME: 08:30
LOCAL: INSTITUTO DE QUÍMICA
TITLE:

TOTAL AND BIOACCESSIBLE CONTENT OF PHENOLIC BIOACTIVES AND INORGANIC CONSTITUENTS IN NUTRITIOUS FLOUR SAMPLES

KEY WORDS:

nutritious flours, phenolic bioactives, inorganic constituents, bioaccessibility, ICP OES, CLAE-DAD

PAGES: 160
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Instrumentação Analítica
SUMMARY:

Phenolic bioactives, present in plant foods, modulate reactive species in the human body and inorganic constituents such as Cu, Fe, Mn, Zn, Ca, K, P participate in vital functions in the body. The aim of this study was to determine the total and bioaccessible contents of phenolic bioactives and inorganic constituents in nutritious flour samples. The bioactive extraction procedure (gallic, protocatechuic, chlorogenic, caffeic, syringic, p-coumaric, sinapic, ellagic acids and the flavonoids catechin, rutin, quercetin and kaempferol) consisted of: 0.25 g sample + 1.5 mL of acetone + 8.5 mL of methanol, in ultrasound at room temperature for 30 min and determination by high-performance liquid chromatography with diode array detector (HPLC-DAD). For inorganic constituents (Al, Ba, Ca, Cu, Fe, K, Na, Mn, Mg, P, Sr and Zn), were used 0.25 g of sample + 7.0 mL of HNO₃ 5.0 mol L ^-1 + 1.0 mL of 30% H₂O₂ (m m^-1), at 200 °C for 45 min, in a digester block with a closed system and determination by inductively coupled plasma optical emission spectrometry (ICP OES). The LOQ of bioactives, in µg g^-1, ranged from 2.76 (ellagic) to 3.90 (catechin) and of inorganic constituents from 0.005 (Na) to 13.94 (Al). To extract the bioaccessible fraction of phenolics and inorganic constituents, the INFOGEST in vitro static simulation was used. The bioactive contents, in µg g^-1, varied between: < 0.17 to 1.37 ± 0.07 (ellagic) and < 0.16 to 88.83 ± 0.72 (catechin). Kaempferol was below the LOQ. For inorganic constituents, in µg g^-1, they varied between: 0.09 ± 0.01 to 6.67 ± 0.77 (Al) and 0.59 ± 0.07 to 32.96 ± 3.28 (Fe). The elements Al, Cu, Fe and Zn were below the LOQ in some flour samples. The bioaccessible fraction ranged from 0.87 to 98.0% in phenolic bioactives and 0.21 to 73.3% in inorganic constituents.

COMMITTEE MEMBERS:
Interno - ***.820.765-** - ANIBAL DE FREITAS SANTOS JUNIOR - UNEB
Interna - 2721446 - DANIELE CRISTINA MUNIZ BATISTA DOS SANTOS
Interna - 2422743 - GISELE ANDRE BAPTISTA CANUTO
Interna - 282814 - MARIA DAS GRACAS ANDRADE KORN
Interno - 1698441 - RENNAN GEOVANNY OLIVEIRA ARAUJO
Externa à Instituição - DÉBORA DE ANDRADE SANTANA - UNEB
Externo à Instituição - MADSON DE GODOI PEREIRA - UNEB
Externa à Instituição - LAÍS ARAÚJO SOUZA - UFPE