Banca de DEFESA: RHAISSA RIBEIRO BARBOSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RHAISSA RIBEIRO BARBOSA
DATE: 17/07/2023
TIME: 09:00
LOCAL: Virtual ou presencial, depende do recurso do programa pra comprar passagens
TITLE:

Physiological and metabolomics evaluation of Ricinus communis seed germination and phylogenetic and functional analysis of overexpressed GDSL esterase/lipase genes in Arabidopsis thaliana

KEY WORDS:

metabolomics;

abiotic stresses;

plasticity

PAGES: 100
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Orgânica
SUMMARY:

Ricinus communis is an important oilseed crop with great socioeconomic impact, and is also essential for small family farmers in semi-arid regions of Brazil and the world. Its resistance to adverse conditions, such as thermal and saline stress and water restriction, is very interesting; as well as the metabolic pathways and genes involved in this resistance to these stresses. Despite this, few studies have been carried out on the metabolic and genetic profile, as well as the possible contribution that these data can provide for the survival of crops in different environmental conditions. R. communis seeds were germinated under thermal stress conditions (35°C) and at the ideal growth temperature (25°C), in periods of five and fifteen days, where several physiological parameters were observed. This action was developed with the aim of better understanding the metabolic profile of R. communis seedlings, through Nuclear Magnetic Resonance (NMR) analysis and multivariate statistical analysis, in addition to proposing which metabolic pathways may be being used by them to resist stress. thermal. Among the genes expressed by R. communis, some stand out for being activated in the face of abiotic stresses. The present work is dedicated to studying the GDSL esterase/lipase gene family; the proteins encoded by this family play an important role in lipid metabolism. Due to their substrate specificity, these enzymes are considered highly promising with potential use for applications in several areas. The GDSL esterase/lipases already studied demonstrate as main physiological functions the role in the regulation of development and growth, morphogenesis and lipid metabolism of plants. Species overexpressing GDSL esterase/lipase genes showed greater resistance to several abiotic and oxidative stresses. Despite the socioeconomic importance of R. communis in semi-arid regions, the characterization of the GDSL esterase/lipase genes and their possible contribution to the survival of R. communis plants was not found in the literature. Thus, this work also aimed to characterize the Ricinus communis GDSL esterase/lipase family (RcGELP), through bioinformatics data, such as gene structure, subcellular location, phylogeny, among others. Furthermore, we analyzed the germination against saline stress and water restriction of Arabidopsis thaliana seeds overexpressing or silencing RcGELP genes in comparison with a wild lineage; in order to understand the role of these genes in plant resistance to abiotic stresses. We also developed a metabolomic analysis of samples of germinated seedlings against the aforementioned stresses in order to understand which metabolites and metabolic pathways are involved in the resistance of these seeds and their relationship with overexpression and/or silencing of RcGELP genes. These results can contribute to genetic improvement programs aimed at the development of plants with high tolerance to abiotic stresses. Providing economic and social support to farmers in semi-arid areas around the world.

COMMITTEE MEMBERS:
Interno - 3768242 - PAULO ROBERTO RIBEIRO DE JESUS
Interna - 1756798 - ELISANGELA FABIANA BOFFO
Interna - 2454639 - CALINE GOMES FERRAZ
Externa ao Programa - 287294 - LUZIMAR GONZAGA FERNANDEZ - UFBAExterno ao Programa - 2085555 - RENATO DELMONDEZ DE CASTRO - UFBAExterno à Instituição - LIV SOARES SEVERINO
Externo à Instituição - ANTONIO GILBERTO FERREIRA